ArticleNature protocols2017
Construction of an aerolysin nanopore in a lipid bilayer for single-oligonucleotide analysis.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed.
- Large-Diameter DNA-Scaffolded Nanopores Enabled by Loosely Packed Peptides for Single-Molecule Sensing.Angewandte Chemie (International ed. in English) · 2026Article
- Intelligent molecular logic computing toolkits: nucleic acid-based construction, functionality, and enhanced biosensing applications.Chemical science · 2025Review
- Redesign of Translocon EXP2 Nanopore for Detecting Peptide Fragments.Small methods · 2025Article
- High-throughput single biomarker identification using droplet nanopore.Chemical science · 2024Article
- Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics.Nature chemistry · 2024Review
- Identification of tagged glycans with a protein nanopore.Nature communications · 2023Article
- Unbiased Data Analysis for the Parameterization of Fast Translocation Events through Nanopores.ACS omega · 2022Article
- Single-molecule analysis of interaction between p53TAD and MDM2 using aerolysin nanopores.Chemical science · 2021Article
- Critical Review: digital resolution biomolecular sensing for diagnostics and life science research.Lab on a chip · 2020Review
- Direct Quantification of Damaged Nucleotides in Oligonucleotides Using an Aerolysin Single Molecule Interface.ACS central science · 2020Article
- Active DNA unwinding and transport by a membrane-adapted helicase nanopore.Nature communications · 2019Article
- Comparative biosensing of glycosaminoglycan hyaluronic acid oligo- and polysaccharides using aerolysin andThe European physical journal. E, Soft matter · 2018Article
- Nanopore label-free detection of single-nucleotide deletion in Baxα/BaxΔ2.Electrophoresis · 2018Article
- The aerolysin nanopore: from peptidomic to genomic applications.Nanoscale · 2018Review
- Mapping the sensing spots of aerolysin for single oligonucleotides analysis.Nature communications · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanopore techniques offer the possibility to study biomolecules at the single-molecule level in a low-cost, label-free and high-throughput manner. By analyzing the level, duration and frequency of ionic current blockades, information regarding the structural conformation, mass, length and concentration of single molecules can be obtained in physiological conditions. Aerolysin monomers assemble into small pores that provide a confined space for effective electrochemical control of a single molecule interacting with the pore, which significantly improves the temporal resolution of this technique. In comparison with other reported protein nanopores, aerolysin maintains its functional stability in a wide range of pH conditions, which allows for the direct discrimination of oligonucleotides between 2 and 10 nt in length and the monitoring of the stepwise cleavage of oligonucleotides by exonuclease I (Exo I) in real time. This protocol describes the process of activating proaerolysin using immobilized trypsin to obtain the aerolysin monomer, the construction of a lipid membrane and the insertion of an individual aerolysin nanopore into this membrane. A step-by-step description is provided of how to perform single-oligonucleotide analyses and how to process the acquired data. The total time required for this protocol is ∼3 d.
Indexed as
Identifiers
28837133What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.