Evidence map›Paper›PMID 28945200›Full record

ArticleThe Journal of clinical investigation2017

Neural precursor cell-secreted TGF-β2 redirects inflammatory monocyte-derived cells in CNS autoimmunity.

Donatella De Feo, Arianna Merlini, Elena Brambilla, Linda Ottoboni, Cecilia Laterza, Ramesh Menon, Sundararajan Srinivasan, Cinthia Farina, Jose Manuel Garcia Manteiga, Erica Butti and 4 more

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of clinical investigation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 53 citations in OpenAlex.

  1. Review
  2. Article
  3. Forced polarisation of microglia by IL-13 is modified by inflammatory and microenvironmental context.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025
    Article
  4. Review
  5. Article
  6. iPSCs engrafted in allogeneic hosts without immunosuppression induce donor-specific tolerance to secondary allografts.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. mCell stem cell · 2025
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Stem Cell Therapies for Progressive Multiple Sclerosis.Frontiers in cell and developmental biology · 2021
    Review
  17. Niche Cells Crosstalk In Neuroinflammation After Traumatic Brain Injury.International journal of biological sciences · 2021
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 3 countries.

Donatella De FeoNeuroimmunology Unit.
Arianna MerliniNeuroimmunology Unit.
Elena BrambillaNeuroimmunology Unit.
Linda OttoboniNeuroimmunology Unit.
Cecilia LaterzaNeuroimmunology Unit.
Ramesh MenonImmunobiology of Neurological Disorders Lab, Institute of Experimental Neurology, Division of Neuroscience, and.
Sundararajan SrinivasanImmunobiology of Neurological Disorders Lab, Institute of Experimental Neurology, Division of Neuroscience, and.
Cinthia FarinaImmunobiology of Neurological Disorders Lab, Institute of Experimental Neurology, Division of Neuroscience, and.
Jose Manuel Garcia ManteigaCenter for Translational Genomics and BioInformatics, San Raffaele Scientific Institute and Vita Salute San Raffaele University, Milan, Italy.
Erica ButtiNeuroimmunology Unit.
Marco BacigaluppiNeuroimmunology Unit.
Giancarlo ComiDepartment of Neurology.
Melanie GreterInstitute of Experimental Immunology, University of Zürich, Zürich, Switzerland.
Gianvito MartinoNeuroimmunology Unit.
Institute of Neuroimmunology of the Slovak Academy of Sciences · SKUniversity of Zurich · CHVita-Salute San Raffaele University · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In multiple sclerosis, the pathological interaction between autoreactive Th cells and mononuclear phagocytes in the CNS drives initiation and maintenance of chronic neuroinflammation. Here, we found that intrathecal transplantation of neural stem/precursor cells (NPCs) in mice with experimental autoimmune encephalomyelitis (EAE) impairs the accumulation of inflammatory monocyte-derived cells (MCs) in the CNS, leading to improved clinical outcome. Secretion of IL-23, IL-1, and TNF-α, the cytokines required for terminal differentiation of Th cells, decreased in the CNS of NPC-treated mice, consequently inhibiting the induction of GM-CSF-producing pathogenic Th cells. In vivo and in vitro transcriptome analyses showed that NPC-secreted factors inhibit MC differentiation and activation, favoring the switch toward an antiinflammatory phenotype. Tgfb2-/- NPCs transplanted into EAE mice were ineffective in impairing MC accumulation within the CNS and failed to drive clinical improvement. Moreover, intrathecal delivery of TGF-β2 during the effector phase of EAE ameliorated disease severity. Taken together, these observations identify TGF-β2 as the crucial mediator of NPC immunomodulation. This study provides evidence that intrathecally transplanted NPCs interfere with the CNS-restricted inflammation of EAE by reprogramming infiltrating MCs into antiinflammatory myeloid cells via secretion of TGF-β2.

Indexed as

AnimalsBrainCell DifferentiationCells, CulturedCytokinesDendritic CellsFemaleImmunomodulationMice, 129 StrainMice, Inbred C57BLMicrogliaMonocytesMultiple SclerosisNeural Stem CellsT-Lymphocytes, Helper-InducerTranscriptomeCytokinesTransforming Growth Factor beta2

Identifiers

PMID28945200
PMCPMC5663358
OpenAlexW2759946228

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.