Evidence mapPaperPMID 28956082Full record

ArticleDiabetologia2018

Enteroendocrine K and L cells in healthy and type 2 diabetic individuals.

Tina Jorsal, Nicolai A Rhee, Jens Pedersen, Camilla D Wahlgren, Brynjulf Mortensen, Sara L Jepsen, Jacob Jelsing, Louise S Dalbøge, Peter Vilmann, Hazem Hassan and 5 more

2 registry-linked trialsOpen access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Diabetologia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 79 papers.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed
9.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05446415 naunknown statusstarted 2020, after this paper: background citation

Evaluation of L-Cell Activity in the Small Intestine as Biliopancreatic Loop Extension in Obese Patients With DM2 Submitted to Roux-en-Y Gastric Bypass

Ran2020Enrolled20Registered outcomes2Posted comparisons0ConditionsSevere Obesity, Type 2 Diabetes Mellitus in ObeseArmsAnalyze basal expression incretin for immunolabeling and mRNA expression glucagon-like secretion peptide-1 (GLP-1) and peptide YY (PYY 3-36) incretins by L cells.
PMID 26048514PMID 35730880PMID 33158945other papers from this trial
Open the trial in the graph
NCT03044860 nacompletednot on this map

Enteroendocrine Cells in Healthy Individuals and Patients With Type 2 Diabetes

TypeinterventionalSponsorUniversity Hospital, Gentofte, CopenhagenRan2011 to 2012Enrolled24ConditionsType2 DiabetesArmsDouble-balloon enteroscopy (DBE) with biopsy retrieval
3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 166 citations in OpenAlex.

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  13. Possible mechanisms of action of glucagon-like peptide-1 receptor agonists on blood pressure beyond body weight.Hypertension research : official journal of the Japanese Society of Hypertension · 2025
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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 7 institutions in 2 countries.

Tina JorsalCenter for Diabetes Research, Gentofte Hospital, University of Copenhagen, Kildegårdsvej 28, DK-2900, Hellerup, Denmark.
Nicolai A RheeCenter for Diabetes Research, Gentofte Hospital, University of Copenhagen, Kildegårdsvej 28, DK-2900, Hellerup, Denmark.
Jens PedersenDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Camilla D WahlgrenCenter for Diabetes Research, Gentofte Hospital, University of Copenhagen, Kildegårdsvej 28, DK-2900, Hellerup, Denmark.
Brynjulf MortensenCenter for Diabetes Research, Gentofte Hospital, University of Copenhagen, Kildegårdsvej 28, DK-2900, Hellerup, Denmark.
Sara L JepsenDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Jacob JelsingGubra ApS, Hørsholm, Denmark.
Louise S DalbøgeGubra ApS, Hørsholm, Denmark.
Peter VilmannEndoscopic Unit, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark.
Hazem HassanEndoscopic Unit, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark.
Jakob W HendelEndoscopic Unit, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark.
Steen S PoulsenDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Jens J HolstDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Tina VilsbøllCenter for Diabetes Research, Gentofte Hospital, University of Copenhagen, Kildegårdsvej 28, DK-2900, Hellerup, Denmark.
Filip K KnopCenter for Diabetes Research, Gentofte Hospital, University of Copenhagen, Kildegårdsvej 28, DK-2900, Hellerup, Denmark. filipknop@dadlnet.dk.
Gentofte Hospital · DKUniversity of Copenhagen · DKGubra (Denmark) · DKHerlev Hospital · DKNovo Nordisk (Denmark) · DKNovo Nordisk Foundation · DKNovo Nordisk (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aims/hypothesisEnteroendocrine K and L cells are pivotal in regulating appetite and glucose homeostasis. Knowledge of their distribution in humans is sparse and it is unknown whether alterations occur in type 2 diabetes. We aimed to evaluate the distribution of enteroendocrine K and L cells and relevant prohormone-processing enzymes (using immunohistochemical staining), and to evaluate the mRNA expression of the corresponding genes along the entire intestinal tract in individuals with type 2 diabetes and healthy participants.

methodsIn this cross-sectional study, 12 individuals with type 2 diabetes and 12 age- and BMI-matched healthy individuals underwent upper and lower double-balloon enteroscopy with mucosal biopsy retrieval from approximately every 30 cm of the small intestine and from seven specific anatomical locations in the large intestine.

resultsSignificantly different densities for cells positive for chromogranin A (CgA), glucagon-like peptide-1, glucose-dependent insulinotropic polypeptide, peptide YY, prohormone convertase (PC) 1/3 and PC2 were observed along the intestinal tract. The expression of CHGA did not vary along the intestinal tract, but the mRNA expression of GCG, GIP, PYY, PCSK1 and PCSK2 differed along the intestinal tract. Lower counts of CgA-positive and PC1/3-positive cells, respectively, were observed in the small intestine of individuals with type 2 diabetes compared with healthy participants. In individuals with type 2 diabetes compared with healthy participants, the expression of GCG and PYY was greater in the colon, while the expression of GIP and PCSK1 was greater in the small intestine and colon, and the expression of PCSK2 was greater in the small intestine. CONCLUSIONS/

interpretationOur findings provide a detailed description of the distribution of enteroendocrine K and L cells and the expression of their products in the human intestinal tract and demonstrate significant differences between individuals with type 2 diabetes and healthy participants.

trial registrationNCT03044860.

Indexed as

AdultAgedChromogranin ACross-Sectional StudiesDiabetes Mellitus, Type 2Enteroendocrine CellsFemaleGastric Inhibitory PolypeptideGastrointestinal TractGlucagon-Like Peptide 1HumansImmunohistochemistryMaleMiddle AgedPeptide YYProprotein Convertase 1Chromogranin AGastric Inhibitory PolypeptideGlucagon-Like Peptide 1Peptide YYProprotein Convertase 1Proprotein Convertase 2Proprotein ConvertasesChromogranin ADouble-balloon enteroscopyEnteroendocrine cellsGlucagon-like peptide-1Glucose-dependent insulinotropic polypeptideImmunohistochemistrymRNA expressionPeptide YYProhormone convertaseType 2 diabetes

Identifiers

PMID28956082
OpenAlexW2757755247

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.