ArticleThe Plant journal : for cell and molecular biology2017
Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.
Article in The Plant journal : for cell and molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Cell-Penetrating Peptide-Based Triple Nanocomplex Enables Efficient Nuclear Gene Delivery in Chlamydomonas reinhardtii.Biotechnology and bioengineering · 2025Article
- Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.Current genetics · 2025Article
- Fine-tuning the N-glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants.Plant biotechnology journal · 2024Article
- CRISPR/Cas9-Mediated Knockout of the Lycopene ε-Cyclase for Efficient Astaxanthin Production in the Green MicroalgaPlants (Basel, Switzerland) · 2024Article
- Subscaling of a cytosolic RNA binding protein governs cell size homeostasis in the multiple fission alga Chlamydomonas.PLoS genetics · 2024Article
- Processed microalgae: green gold for tissue regeneration and repair.Theranostics · 2024Review
- A cell-based model for size control in the multiple fission alga Chlamydomonas reinhardtii.Current biology : CB · 2023Article
- A Blue Light-Responsive Strong Synthetic Promoter Based on Rational Design inInternational journal of molecular sciences · 2023Article
- A conserved RWP-RK transcription factor VSR1 controls gametic differentiation in volvocine algae.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Current Nuclear Engineering Strategies in the Green MicroalgaLife (Basel, Switzerland) · 2023Review
- Promoting Photosynthetic Production of Dammarenediol-II inInternational journal of molecular sciences · 2023Article
- Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility.Molecular biology of the cell · 2023Article
- Advanced pathway engineering for phototrophic putrescine production.Plant biotechnology journal · 2022Article
- The Spermidine Synthase GeneCells · 2022Article
- Transformation ofMicroorganisms · 2022Article
- Molecular Advancements Establishing Chlamydomonas as a Host for Biotechnological Exploitation.Frontiers in plant science · 2022Review
- Exploring the Impact of Terminators on Transgene Expression inLife (Basel, Switzerland) · 2021Article
- A recombineering pipeline to clone large and complex genes in Chlamydomonas.The Plant cell · 2021Article
- Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically TransformedLife (Basel, Switzerland) · 2020Article
- Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii.PLoS genetics · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydomonas reinhardtii is a unicellular green alga that has attracted interest due to its potential biotechnological applications, and as a model for algal biofuel and energy metabolism. Despite all the advantages that this unicellular alga offers, poor and inconsistent expression of nuclear transgenes remains an obstacle for basic and applied research. We used a data-mining strategy to identify highly expressed genes in Chlamydomonas whose flanking sequences were tested for the ability to drive heterologous nuclear transgene expression. Candidates identified in this search included two ribosomal protein genes, RPL35a and RPL23, and ferredoxin, FDX1, whose flanking regions including promoters, terminators and untranslated sequences could drive stable luciferase transgene expression to significantly higher levels than the commonly used Hsp70A-RBCS2 (AR) hybrid promoter/terminator sequences. The RPL23 flanking sequences were further tested using the zeocin resistance gene sh-ble as a reporter in monocistronic and dicistronic constructs, and consistently yielded higher numbers of zeocin-resistant transformants and higher levels of resistance than AR- or PSAD-based vectors. Chlamydomonas RPL23 sequences also enabled transgene expression in Volvox carteri. Our study provides an additional benchmark for strong constitutive expression of transgenes in Chlamydomonas, and develops a general approach for identifying flanking sequences that can be used to drive transgene expression for any organism where transcriptome data are available.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.