Evidence map›Paper›PMID 29019804›Full record

ArticleSpine2018

Quantification of Propionic Acid in the Bovine Spinal Disk After Infection of the Tissue With Propionibacteria acnes Bacteria.

Sergey Magnitsky, Stefan Dudli, Xinyan Tang, Jaskanwaljeet Kaur, Joycelyn Diaz, Steve Miller, Jeffrey C Lotz

Open access · greenAbstract read
In one paragraph

Article in Spine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.

  1. The bacteria-positive proportion in the disc tissue samples from surgery: a systematic review and meta-analysis.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2019
    Pooled it
  2. Article
  3. Article
  4. Magnetic resonance spectroscopy (MRS) identification of chemically painful lumbar discs leads to improved 6-, 12-, and 24-month outcomes for discogenic low back pain surgeries.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2023
    Article
  5. Review
  6. Article
  7. Review
  8. Is IL-1β Further Evidence for the Role ofFrontiers in cellular and infection microbiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Sergey MagnitskyDepartment of Radiology, University of California at San Francisco, San Francisco, CA.
Stefan DudliDepartment of Orthopedic Surgery, University of California San Francisco, San Francisco, CA.
Xinyan TangDepartment of Orthopedic Surgery, University of California San Francisco, San Francisco, CA.
Jaskanwaljeet KaurDepartment of Radiology, University of California at San Francisco, San Francisco, CA.
Joycelyn DiazDepartment of Laboratory Medicine, University of California San Francisco, San Francisco, CA.
Steve MillerDepartment of Laboratory Medicine, University of California San Francisco, San Francisco, CA.
Jeffrey C LotzDepartment of Orthopedic Surgery, University of California San Francisco, San Francisco, CA.
University of California, San Francisco · USUniversitätsklinik Balgrist · CH

Funding

UCSF Core Center for Musculoskeletal Biology and MedicineP30AR066262 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MAJUMDAR, SHARMILA · 2014 to 2018
$3.0M
Phenotypes of pathologic vertebral endplate degenerationR01AR063705 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOTZ, JEFFREY C. · 2013 to 2017
$3.0M
Phenotypes of pathologic vertebral endplate degenerationR56AR063705 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOTZ, JEFFREY C. · 2019 to 2019
$669k
NIAMS NIH HHS P30 AR066262NIAMS NIH HHS R01 AR063705NIAMS NIH HHS R56 AR063705
6 · The paper itself

Abstract

STUDY

designResearch.

objectiveThe goal of this study was to investigate whether Propionibacteria acnes infection of the intervertebral disc can be detected noninvasively by nuclear magnetic resonance (NMR) spectroscopy. SUMMARY OF BACKGROUND DATA: Microbiological studies of surgical samples suggest that a significant subpopulation of back pain patients may have occult disc infection with P. acnes bacteria. This hypothesis is further supported by a double-blind clinical trial showing that back pain patients with Modic type 1 changes may respond to antibiotic treatment. Because significant side effects are associated with antibiotic treatment, there is a need for a noninvasive method to detect whether specific discs in back pain patients are infected with P acnes bacteria.

methodsP. acnes bacteria were obtained from human patients. NMR detection of a propionic acid (PA) in the bacteria extracts was conducted on 500 MHz high-resolution spectrometer, whereas in vivo NMR spectroscopy of an isolated bovine disk tissue infected with P. acnes was conducted on 7 T magnetic resonance imaging scanner.

resultsNMR spectra of P. acnes metabolites revealed a distinct NMR signal with identical chemical shits (1.05 and 2.18 ppm) as PA (a primary P. acne metabolite). The 1.05 ppm signal does not overlap with other bacteria metabolites, and its intensity increases linearly with P. acnes concentration. Bovine disks injected with P. acnes bacteria revealed a very distinct NMR signal at 1.05 ppm, which linearly increased with P. acnes concentration.

conclusionThe 1.05 ppm NMR signal from PA can be used as a marker of P. acnes infection of discs. This signal does not overlap with other disc metabolites and linearly depends on P. acnes concentration. Consequently, NMR spectroscopy may provide a noninvasive method to detect disc infection in the clinical setting. LEVEL OF EVIDENCE: N/A.

Indexed as

Propionibacterium acnesAnimalsBack PainBiomarkersCattleGram-Positive Bacterial InfectionsHumansIntervertebral DiscMagnetic Resonance ImagingMagnetic Resonance SpectroscopyPropionatesBiomarkersPropionatespropionic acid

Identifiers

PMID29019804
PMCPMC5893447
OpenAlexW2809382320

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.