ArticlePLoS biology2017
A molecular atlas of the developing ectoderm defines neural, neural crest, placode, and nonneural progenitor identity in vertebrates.
Article in PLoS biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Catalytic activity of KMT5B promotes ciliogenesis without affecting global chromatin accessibility.Life science alliance · 2026Article
- Adam13 interacts with large protein complexes to regulate histone modification and gene expression.Frontiers in cell and developmental biology · 2026Article
- Untangling Zebrafish Genetic Annotation: Addressing Complexities and Nomenclature Issues in Orthologous Evaluation of TCOF1 and NOLC1.Journal of molecular evolution · 2024Article
- Immune-Molecular Link between Thyroid and Skin Autoimmune Diseases: A Narrative Review.Journal of clinical medicine · 2024Review
- Shared features of blastula and neural crest stem cells evolved at the base of vertebrates.Nature ecology & evolution · 2024Article
- SoxB1 transcription factors are essential for initiating and maintaining neural plate border gene expression.Development (Cambridge, England) · 2024Article
- A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Small molecule-mediated reprogramming of Xenopus blastula stem cells to a neural crest state.Developmental biology · 2024Article
- The sulfotransferase XB5850668.L is required to apportion embryonic ectodermal domains.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- A ubiquitin-based effector-to-inhibitor switch coordinates early brain, craniofacial, and skin development.Nature communications · 2023Article
- Quantitative analysis of transcriptome dynamics provides novel insights into developmental state transitions.BMC genomics · 2022Article
- Tissue Rotation of theJournal of developmental biology · 2022Article
- Time to go: neural crest cell epithelial-to-mesenchymal transition.Development (Cambridge, England) · 2022Review
- PFKFB4 interacts with ICMT and activates RAS/AKT signaling-dependent cell migration in melanoma.Life science alliance · 2022Article
- Repressive Interactions Between Transcription Factors Separate Different Embryonic Ectodermal Domains.Frontiers in cell and developmental biology · 2022Article
- Review
- Neural crest multipotency and specification: power and limits of single cell transcriptomic approaches.Faculty reviews · 2021Review
- The vertebrate-specific VENTX/NANOG gene empowers neural crest with ectomesenchyme potential.Science advances · 2020Article
- Insights Into the Early Gene Regulatory Network Controlling Neural Crest and Placode Fate Choices at the Neural Border.Frontiers in physiology · 2020Review
- Evolutionary and Developmental Associations of Neural Crest and Placodes in the Vertebrate Head: Insights From Jawless Vertebrates.Frontiers in physiology · 2020Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
During vertebrate neurulation, the embryonic ectoderm is patterned into lineage progenitors for neural plate, neural crest, placodes and epidermis. Here, we use Xenopus laevis embryos to analyze the spatial and temporal transcriptome of distinct ectodermal domains in the course of neurulation, during the establishment of cell lineages. In order to define the transcriptome of small groups of cells from a single germ layer and to retain spatial information, dorsal and ventral ectoderm was subdivided along the anterior-posterior and medial-lateral axes by microdissections. Principal component analysis on the transcriptomes of these ectoderm fragments primarily identifies embryonic axes and temporal dynamics. This provides a genetic code to define positional information of any ectoderm sample along the anterior-posterior and dorsal-ventral axes directly from its transcriptome. In parallel, we use nonnegative matrix factorization to predict enhanced gene expression maps onto early and mid-neurula embryos, and specific signatures for each ectoderm area. The clustering of spatial and temporal datasets allowed detection of multiple biologically relevant groups (e.g., Wnt signaling, neural crest development, sensory placode specification, ciliogenesis, germ layer specification). We provide an interactive network interface, EctoMap, for exploring synexpression relationships among genes expressed in the neurula, and suggest several strategies to use this comprehensive dataset to address questions in developmental biology as well as stem cell or cancer research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.