ArticleSurgery2018
Microparticles from stored red blood cells promote a hypercoagulable state in a murine model of transfusion.
Article in Surgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Association of erythropoiesis-stimulating agents and red blood cell transfusion with venous thrombosis in patients with cancer: a nationwide case-crossover study.Annals of medicine · 2026Article
- Erythrocytes as immunomodulators in trauma: orchestrating clearance pathways, inflammation, and organ injury.Frontiers in immunology · 2026Review
- Extracellular vesicles in ageing cold-stored whole blood may not compensate for the decreasing haemostatic function in vitro.Transfusion medicine (Oxford, England) · 2025Article
- Amitriptyline Decreases Mouse Lung Endothelial Cell Inflammatory Responses to Packed Red Blood Cell Microparticles.The Journal of surgical research · 2024Article
- Porcine Packed Red Blood Cells Demonstrate a Distinct Red Blood Cell Storage Lesion.The Journal of surgical research · 2024Article
- Microvesicles from stored red blood cells induce P-selectin and von Willebrand factor release from endothelial cells via a protein kinase C-dependent mechanism.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2024Article
- Red Blood Cell Contribution to Thrombosis in Polycythemia Vera and Essential Thrombocythemia.International journal of molecular sciences · 2024Review
- Red Blood Cell-Related Parameters in Rheumatoid Arthritis: Clinical Value and Immunological Significance.Journal of inflammation research · 2024Review
- Red Blood Cell-Derived Extracellular Vesicles: An Overview of Current Research Progress, Challenges, and Opportunities.Biomedicines · 2023Review
- Hematological alterations associated with long COVID-19.Frontiers in physiology · 2023Review
- Red blood cell-derived phosphatidylserine positive extracellular vesicles are associated with past thrombotic events in patients with systemic erythematous lupus.Lupus science & medicine · 2022Article
- Effect of Leukoreduction on Hematobiochemical Parameters and Storage Hemolysis in Canine Whole Blood Units.Animals : an open access journal from MDPI · 2021Article
- Processing methods and storage duration impact extracellular vesicle counts in red blood cell units.Blood advances · 2020Article
- Innate coagulability changes with age in stored packed red blood cells.Thrombosis research · 2020Article
- Article
- Microparticles from aged packed red blood cell units stimulate pulmonary microthrombus formation via P-selectin.Thrombosis research · 2020Article
- Red blood cells: the forgotten player in hemostasis and thrombosis.Journal of thrombosis and haemostasis : JTH · 2019Review
- Endothelial Extracellular Vesicles in Pulmonary Function and Disease.Current topics in membranes · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundRed blood cell-derived microparticles are biologically active, submicron vesicles shed by erythrocytes during storage. Recent clinical studies have linked the duration of red blood cell storage with thromboembolic events in critically ill transfusion recipients. In the present study, we hypothesized that microparticles from aged packed red blood cell units promote a hypercoagulable state in a murine model of transfusion.
methodsMicroparticles were isolated from aged, murine packed red blood cell units via serial centrifugation. Healthy male C57BL/6 mice were transfused with microparticles or an equivalent volume of vehicle, and whole blood was harvested for analysis via rotational thromboelastometry. Serum was harvested from a separate set of mice after microparticles or saline injection, and analyzed for fibrinogen levels. Red blood cell-derived microparticles were analyzed for their ability to convert prothrombin to thrombin. Finally, mice were transfused with either red blood cell microparticles or saline vehicle, and a tail bleeding time assay was performed after an equilibration period of 2, 6, 12, or 24 hours.
resultsMice injected with red blood cell-derived microparticles demonstrated an accelerated clot formation time (109.3 ± 26.9 vs 141.6 ± 28.2 sec) and increased α angle (68.8 ± 5.0 degrees vs 62.8 ± 4.7 degrees) compared with control (each P < .05). Clotting time and maximum clot firmness were not significantly different between the 2 groups. Red blood cell-derived microparticles exhibited a hundredfold greater conversion of prothrombin substrate to its active thrombin form (66.60 ± 0.03 vs 0.70 ± 0.01 peak OD; P<.0001). Additionally, serum fibrinogen levels were lower in microparticles-injected mice compared with saline vehicle, suggesting thrombin-mediated conversion to insoluble fibrin (14.0 vs 16.5 µg/mL, P<.05). In the tail bleeding time model, there was a more rapid cessation of bleeding at 2 hours posttransfusion (90.6 vs 123.7 sec) and 6 hours posttransfusion (87.1 vs 141.4 sec) in microparticles-injected mice as compared with saline vehicle (each P<.05). There was no difference in tail bleeding time at 12 or 24 hours.
conclusionRed blood cell-derived microparticles induce a transient hypercoagulable state through accelerated activation of clotting factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.