Ymr210wp leads to the accumulation of phospholipids and steryl esters in yeast.

Naresh Kumar Manda, Venkata Bala Sai Chaitanya Thunuguntla, Chandrasekhar Bokka, Bondili Jayakumar Singh

Open access · hybridAbstract read

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Article in Bioinformation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 43% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Naresh Kumar MandaDepartment of Biotechnology, K L University, Green fields, Vaddeswaram, Guntur Dist, Andhra Pradesh, India - 522502.

Venkata Bala Sai Chaitanya ThunuguntlaDepartment of Biotechnology, K L University, Green fields, Vaddeswaram, Guntur Dist, Andhra Pradesh, India - 522502.

Chandrasekhar BokkaDepartment of Biotechnology, K L University, Green fields, Vaddeswaram, Guntur Dist, Andhra Pradesh, India - 522502.

Bondili Jayakumar SinghDepartment of Biotechnology, K L University, Green fields, Vaddeswaram, Guntur Dist, Andhra Pradesh, India - 522502.

Koneru Lakshmaiah Education Foundation · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ymr210w was identified as a MAG (Monoacylglycerol) lipase. The accumulation of the phospholipids in the ymr210wΔ was not clearly understood. It was expressed in S. cerevisiae using pYES2/CT vector and His-tag purified recombinant protein confirmed TAG lipase activity. To further evaluate the role of YMR210w, ester hydrolase activity was also confirmed with pNP-acetate, pNP-butyrate and pNP - palmitate. GC-MS lipid profiling of ymr210wΔ showed an increase in the 15:0 Pentadecanoic acid by 76% among the total lipids. Phospholipid, Erucic acid 22:1 (Δ13) showed 43% increase while steryl esters showed significant changes with 16:0 hexadecanoic acid augmentations by 80% and 18:0 Octadecanoic acid by 165% when compared to wild type (WT). Increase in the steryl ester and TAG content supports the accumulation of lipid bodies in ymr210wΔ strain when compared with WT cells.

Indexed as

Ester HydrolaseLipid dropletsSaccharomyces cerevisiaeSteryl estersTAG LipaseTriacylglycerols

Identifiers

PMID29225428

DOI10.6026/97320630013360

PMCPMC5712780

OpenAlexW2768418212

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.