ArticleThe journal of trauma and acute care surgery2018
Microparticles formed during storage of red blood cell units support thrombin generation.
Article in The journal of trauma and acute care surgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Impact of the production method on the properties of erythrocyte-derived extracellular particles: a quantitative and qualitative evaluation.Nanotheranostics · 2025Article
- New and emerging technologies for pretransfusion blood quality assessment: A state-of-the-art review.Transfusion · 2024Review
- Changes in hemoglobin oxidation and band 3 during blood storage impact oxygen sensing and mitochondrial bioenergetic pathways in the human pulmonary arterial endothelial cell model.Frontiers in physiology · 2023Article
- Red Blood Cell-Derived Microparticles Exert No Cancer Promoting Effects on Colorectal Cancer Cells In Vitro.International journal of molecular sciences · 2022Article
- Blood Cell-Derived Microvesicles in Hematological Diseases and beyond.Biomolecules · 2022Review
- Storage conditions determine the characteristics of red blood cell derived extracellular vesicles.Scientific reports · 2022Article
- Whole Blood Thrombin Generation in Severely Injured Patients Requiring Massive Transfusion.Journal of the American College of Surgeons · 2021Article
- Bedside Allogeneic Erythrocyte Washing with a Cell Saver to Remove Cytokines, Chemokines, and Cell-derived Microvesicles.Anesthesiology · 2021Observational
- Red blood cell transfusion does not increase risk of venous or arterial thrombosis during hospitalization.American journal of hematology · 2021Article
- Article
- Influence of factor XII deficiency on activated partial thromboplastin time (aPTT) in critically ill patients.Journal of thrombosis and thrombolysis · 2019Article
- Washing or filtering of blood products does not improve outcome in a rat model of trauma and multiple transfusion.Transfusion · 2019Article
- Anaemia requiring red blood cell transfusion is associated with unfavourable 90-day survival in surgical patients with sepsis.BMC research notes · 2018Observational
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIntact red blood cells (RBCs) appear to support thrombin generation in in vitro models of blood coagulation. During storage of RBC units, biochemical, structural, and physiological changes occur including alterations to RBC membranes and release of microparticles, which are collectively known as storage lesion. The clinical consequences of microparticle formation in RBC units are unclear. This study was performed to assess thrombin generation via the prothrombinase complex by washed RBCs and RBC-derived microparticles as a function of RBC unit age.
methodsWell-characterized kinetic and flow cytometric assays were used to quantify and characterize microparticles isolated from leukocyte-reduced RBC units during storage for 42 days under standard blood banking conditions.
resultsStored RBCs exhibited known features of storage lesion including decreasing pH, cell lysis, and release of microparticles demonstrated by scanning electron microscopy. The rate of thrombin formation by RBC units linearly increased during storage, with the microparticle fraction accounting for approximately 70% of the prothrombinase activity after 35 days. High-resolution flow cytometric analyses of microparticle isolates identified phosphatidylserine-positive RBC-derived microparticles; however, their numbers over time did not correlate with thrombin formation in that fraction.
conclusionRed blood cell-derived microparticles capable of supporting prothrombinase function accumulate during storage, suggesting an increased potential of transfused units as they age to interact in unplanned ways with ongoing hemostatic processes in injured individuals, especially given the standard blood bank practice of using the oldest units available.
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Registered trials
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