Evidence map›Paper›PMID 29256222›Full record

ArticleZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences2017

[Effect of microRNA-29b on proliferation and migration of breast cancer cells and its molecular mechanism].

Yiqian Jiang, Qingmin Guo, Jianzhong Gu, Xiaoping Xu, Suhong An, Fang Su, Yanhong Bao, Changxin Huang, Xiaoxiang Guan

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yiqian JiangJinling Clinical Medical College, Nanjing Medical University, Nanjing 210002, China.
Qingmin GuoCenter of Oncology, Xiaoshan First people's Hospital Affiliated to the Medical College, Hangzhou Normal University, Hangzhou 311200, China.
Jianzhong GuDepartment of Oncology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China.
Xiaoping XuCenter of Oncology, Xiaoshan First people's Hospital Affiliated to the Medical College, Hangzhou Normal University, Hangzhou 311200, China.
Suhong AnCenter of Oncology, Xiaoshan First people's Hospital Affiliated to the Medical College, Hangzhou Normal University, Hangzhou 311200, China.
Fang SuCenter of Oncology, Xiaoshan First people's Hospital Affiliated to the Medical College, Hangzhou Normal University, Hangzhou 311200, China.
Yanhong BaoCenter of Oncology, Xiaoshan First people's Hospital Affiliated to the Medical College, Hangzhou Normal University, Hangzhou 311200, China.
Changxin HuangCenter of Oncology, Xiaoshan First people's Hospital Affiliated to the Medical College, Hangzhou Normal University, Hangzhou 311200, China.
Xiaoxiang GuanJinling Clinical Medical College, Nanjing Medical University, Nanjing 210002, China. xxguan@hotmail.com.
Hangzhou Normal University · CNNanjing Medical University · CNZhejiang Chinese Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effects of microRNA(miRNA)-29b on the proliferation and migration of breast cancer cells and its molecular mechanism.

methodsThe recombinant lentiviral expression vector (lenti-miRNA-29b) was constructed and transfected into 293T cells to obtain lentivirus particles that were used to infect breast cancer MCF-7 cells. Transfection efficiency of lenti-miRNA-29b in MCF-7 cells was identified by the expression of green fluorescent protein (GFP). The expression of miRNA-29b was detected by real-time PCR. The cell proliferation and migration were detected by CCK8 assay and Transwell assay, respectively. The bioinformatics softwares were used to predict and screen the downstream target genes regulated by miRNA-29b, which were verified by double luciferase reporter gene assay, RT-PCR and Western blot. The effects of screened target gene RTKN on the growth and migration of MCF-7 cells were verified by RTKN siRNA.

resultsRecombinant lentiviral expression vector of miRNA-29b were successfully constructed. About 90% and 60% of the breast cancer cells showed green fluorescence in lenti-miRNA-29b and lenti-miRNA-NC groups, respectively. The expression of miRNA-29b in lenti-miRNA-29b group increased significantly compared with the lenti-miRNA-NC group and blank control group (all

conclusionsMiRNA-29b can inhibit the proliferation, invasion and metastasis of breast cancer cells by inhibiting the expression of RTKN.

Indexed as

Cell MovementCell ProliferationGene Expression Regulation, NeoplasticMicroRNAsBreast NeoplasmsCell Line, TumorHumansIn Vitro TechniquesMCF-7 CellsMicroRNAs

Identifiers

PMID29256222
PMCPMC10396902
OpenAlexW2977668641

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.