ArticleJournal of experimental botany2018
Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 57 citations in OpenAlex.
- The chimeric gene orf610a reduces cotton pollen fertility by impairing the assembly of ATP synthase.Plant biotechnology journal · 2025Article
- Two novel alleles of the MYB transcription factorHorticulture research · 2025Article
- Single-cell transcriptomic and cell‑type‑specific regulatory networks in Polima temperature-sensitive cytoplasmic male sterility of Brassica napus L.BMC plant biology · 2024Article
- Genome wide identification of BjSWEET gene family and drought response analysis of BjSWEET12 and BjSWEET17 genes in Brassica juncea.BMC plant biology · 2024Article
- Review
- A CRISPR/Cas9-induced male-sterile line facilitating easy hybrid production in polyploid rapeseed (Horticulture research · 2024Article
- Cloning and Expression Analysis of Onion (Allium cepa L.) MADS-Box Genes and Regulation Mechanism of Cytoplasmic Male Sterility.Biochemical genetics · 2023Article
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil theInternational journal of molecular sciences · 2022Article
- Article
- A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- High-generation near-isogenic lines combined with multi-omics to study the mechanism of polima cytoplasmic male sterility.BMC plant biology · 2021Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- Elucidating Mitochondrial DNA Markers ofFrontiers in plant science · 2021Article
- Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation inFrontiers in genetics · 2020Article
- Differentially Expressed Genes between Carrot Petaloid Cytoplasmic Male Sterile and Maintainer during Floral Development.Scientific reports · 2019Article
- Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Mitochondrial genome and transcriptome analysis of five alloplasmic male-sterile lines in Brassica juncea.BMC genomics · 2019Article
- Comparative proteomic analysis of multi-ovary wheat under heterogeneous cytoplasm suppression.BMC plant biology · 2019Article
- Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea.Journal of experimental botany · 2018Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility (CMS) is primarily caused by chimeric genes located in the mitochondrial genomes. In Brassica juncea, orf288 has been identified as a CMS-associated gene in the hau CMS line; however, neither the specific abortive stage nor the molecular function of the gene have been determined. We therefore characterized the hau CMS line, and found that defective mitochondria affect the development of archesporial cells during the L2 stage, leading to male sterility. The expression level of the orf288 transcript was higher in the male-sterility line than in the fertility-restorer line, although no significant differences were apparent at the protein level. The toxicity region of ORF288 was found to be located near the N-terminus and repressed growth of Escherichia coli. However, transgenic expression of different portions of ORF288 indicated that the region that causes male sterility resides between amino acids 73 and 288, the expression of which in E. coli did not result in growth inhibition. Transcriptome analysis revealed a wide range of genes involved in anther development and mitochondrial function that were differentially expressed in the hau CMS line. This study provides new insights into the hau CMS mechanism by which orf288 affects the fertility of Brassica juncea.
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