ArticleThe Analyst2018
Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).
Article in The Analyst, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- SpotOn: A Tool to Enhance Echo Acoustic Standard Deposition for Quantitative Mass Spectrometry Imaging.Chemical & biomedical imaging · 2026Article
- Demonstrating Voxel-by-Voxel (V × V) Single-Point Calibration in Whole-Body Zebrafish by IR-MALDESI Quantitative MSI.Journal of mass spectrometry : JMS · 2026Article
- Exploring ammonium salt doping to enhance ion abundance for quantitative mass spectrometry imaging.Analytical and bioanalytical chemistry · 2025Article
- Demonstrating voxel-by-voxel (V × V) single-point calibration in liver tissue by IR-MALDESI quantitative MSI.Analytical and bioanalytical chemistry · 2025Article
- Branched-Chain Amino Acid Catabolism Promotes Ovarian Cancer Cell Proliferation via Phosphorylation of mTOR.Cancer research communications · 2025Article
- Quantitative sampling by IR-MALDESI is not susceptible to tissue heterogeneity in a multi-organ model.Analytical and bioanalytical chemistry · 2025Article
- Branched-chain amino acid catabolism promotes ovarian cancer cell proliferation via phosphorylation of mTOR.bioRxiv : the preprint server for biology · 2024Article
- Absolute Quantification of Glutathione Using Top-Hat Optics for IR-MALDESI Mass Spectrometry Imaging.Journal of mass spectrometry : JMS · 2024Article
- Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI.Journal of the American Society for Mass Spectrometry · 2024Article
- Challenges and recent advances in quantitative mass spectrometry-based metabolomics.Analytical science advances · 2024Review
- Quantitative mass spectrometry imaging (qMSI): A tutorial.Journal of mass spectrometry : JMS · 2024Article
- Recent Developments and Application of Mass Spectrometry Imaging in N-Glycosylation Studies: An Overview.Mass spectrometry (Tokyo, Japan) · 2024Review
- The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.Mass spectrometry reviews · 2023Review
- Evaluation of Quantitative Platforms for Single Target Mass Spectrometry Imaging.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Multimodal Mass Spectrometry Imaging of Rat Brain Using IR-MALDESI and NanoPOTS-LC-MS/MS.Journal of proteome research · 2022Article
- ADVANCES IN HIGH-RESOLUTION MALDI MASS SPECTROMETRY FOR NEUROBIOLOGY.Mass spectrometry reviews · 2022Review
- Quantitative Mass Spectrometry Imaging with Liquid Microjunction Surface Sampling.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Models for measuring metabolic chemical changes in the metastasis of high grade serous ovarian cancer: fallopian tube, ovary, and omentum.Molecular omics · 2021Review
- Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization.Metabolites · 2021Article
- Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis.Metabolites · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A quantitative mass spectrometry imaging (QMSI) method for absolute quantification of glutathione (GSH) in healthy and cancerous hen ovarian tissues using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is presented. Using this technique, the ion abundance of GSH was normalized to that of a structural analogue, which was sprayed on the slide prior to mounting the tissue sections. This normalization strategy significantly improved the voxel-to-voxel variability; the variability is attributed to the overall ionization process. Subsequently, a series of calibration spots of stable isotope-labeled (SIL) GSH were pipetted on top of the tissue to construct a spatial calibration curve, and calculate the concentration of GSH in both tissue sections. The QMSI results were verified by LC-MS/MS quantification of GSH for the same tissues. GSH was extracted from tissue sections in a slightly acidic buffer and was then alkylated using N-ethylmaleimide to minimize autoxidation of GSH to glutathione disulfide. The alkylated GSH was separated from other contaminants using reversed phase liquid chromatography (RPLC) coupled to a triple quadrupole mass spectrometer, and the z-ion transition of NEM-GSH was used to quantify GSH in each tissue section. While the absolute values obtained using IR-MALDESI QMSI and LC-MS/MS were different, a ∼2-fold increase in the concentration of GSH in cancer tissue compared to the healthy tissue was observed using both techniques. Possible reasons for the difference between absolute concentration values obtained using IR-MALDESI QMSI and LC-MS/MS are also discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.