Evidence map›Paper›PMID 29334206›Full record

ArticleInternational journal of fertility & sterility2018

Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome.

Zahra Shaaban, Mohammad Reza Jafarzadeh Shirazi, Mohammad Hossein Nooranizadeh, Amin Tamadon, Farhad Rahmanifar, Somayeh Ahmadloo, Amin Ramezani, Mohammad Javad Zamiri, Iman Razeghian Jahromi, Fatemeh Sabet Sarvestani and 1 more

Open access · greenAbstract read
In one paragraph

Article in International journal of fertility & sterility, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. New Insights in Bovine Follicular Cysts.Reproduction in domestic animals = Zuchthygiene · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Zahra ShaabanDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran.
Mohammad Reza Jafarzadeh ShiraziDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran. Electronic address: jafarzd@shirazu.ac.ir.
Mohammad Hossein NooranizadehStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Amin TamadonStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Farhad RahmanifarDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Somayeh AhmadlooStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Amin RamezaniDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran.
Mohammad Javad ZamiriDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran.
Iman Razeghian JahromiCardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Fatemeh Sabet SarvestaniStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Omid Koohi HosseinabadiComparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Shiraz University of Medical Sciences · IRShiraz University · IRStem Cell Technology Research Center · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAn abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide (RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in mammalian hypothalamus. The current study performed in order to investigate the expression of RFRP3 mRNA in the dorsomedial hypothalamic nucleus (DMH) after the induction of PCOS in a rat model of constant light exposure, and the possible role of parity on occurrence of PCOS. MATERIALS AND

methodsIn the experimental study, female nulliparous (n=12) and primiparous (n=12) rats were randomly subdivided into control and PCOS subgroups (n=6). PCOS were induced by 90 days exposure to constant light. After 90 days, blood, brain, and ovaries were sampled. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were evaluated. In addition, six adult female ovariectomized rats as a control of real-time polymerase chain reaction (PCR) tests were prepared and in the DMH of all rats, the relative mRNA expression of RFRP3 was assessed.

resultsHistological evaluation of ovaries represented the polycystic features. In addition, serum concentrations of testosterone in the PCOS subgroups were more than the controls (P<0.05). Furthermore, the relative expression of RFRP3 mRNA in PCOS subgroups was lower than the controls (P<0.05).

conclusionConstant light model of the PCOS-induced rats decreased the gene expression of RFRP3 in the DMH that suggests the decrease of RFRP3 may reduce its inhibitory effect on GnRH during the PCOS pathogenesis. This effect was stronger in the nulliparous rats than the primiparous.

Indexed as

Constant LightDorsomedial Hypothalamic NucleusPolycystic Ovary SyndromeRatsRFamide-Related Peptide-3

Identifiers

PMID29334206
PMCPMC5767931
OpenAlexW3023457302

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.