Evidence map›Paper›PMID 29348472›Full record

ArticleScientific reports2018

Viral highway to nucleus exposed by image correlation analyses.

Elina Mäntylä, Jenu V Chacko, Vesa Aho, Colin R Parrish, Victor Shahin, Michael Kann, Michelle A Digman, Enrico Gratton, Maija Vihinen-Ranta

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Nuclear entry and egress of parvoviruses.Molecular microbiology · 2022
    Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Elina MäntyläDepartment of Biological and Environmental Science and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland.ORCID http://orcid.org/0000-0002-8681-8772
Jenu V ChackoLaboratory for Fluorescence Dynamics, University of California, Irvine, California, USA.
Vesa AhoDepartment of Physics, and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, FI-40500, Finland.
Colin R ParrishBaker Institute for Animal Health, College of Veterinary Medicine, University of Cornell, Ithaca, USA.
Victor ShahinInstitute of Physiology II, University of Münster, Münster, Germany.
Michael KannUniversity of Bordeaux, Microbiologie Fondamentale et Pathogénicité, Bordeaux, France.
Michelle A DigmanLaboratory for Fluorescence Dynamics, University of California, Irvine, California, USA.
Enrico GrattonLaboratory for Fluorescence Dynamics, University of California, Irvine, California, USA.
Maija Vihinen-RantaDepartment of Biological and Environmental Science and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland. maija.vihinen-ranta@jyu.fi.
Fluor (United States) · USUniversity of Jyväskylä · FICornell University · USUniversité de Bordeaux · FRUniversity of Münster · DE

Funding

Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING · 2007 to 2016
$25.1M
TRD4: Phasor analysisP41GM103540 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GRATTON, ENRICO · 2012 to 2020
$12.5M
NIGMS NIH HHS P41 GM103540NIGMS NIH HHS P50 GM076516
6 · The paper itself

Abstract

Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm. The unique combination of image analyses allowed us to follow the fate of intracellular single virus particles and their interactions with importin β revealing previously unknown dynamics of the entry pathway.

Indexed as

Active Transport, Cell NucleusAnimalsbeta KaryopherinsCapsidCapsid ProteinsCatsCell LineCell NucleusCytosolEpithelial CellsFluorescent DyesGene ExpressionGreen Fluorescent ProteinsImage Processing, Computer-AssistedMicroscopy, Atomic ForceMicroscopy, ConfocalAlexa594beta KaryopherinsCapsid Proteinsenhanced green fluorescent proteinFluorescent DyesGreen Fluorescent ProteinsOrganic Chemicals

Identifiers

PMID29348472
PMCPMC5773500
OpenAlexW2783322666

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.