ArticleMetabolomics : Official journal of the Metabolomic Society2018
High-throughput extraction and quantification method for targeted metabolomics in murine tissues.
Article in Metabolomics : Official journal of the Metabolomic Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 97 citations in OpenAlex.
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- Metabolomic Profiling of Oral Potentially Malignant Disorders and Its Clinical Values.Biomedicines · 2024Review
- Liver transcriptomics-metabolomics integration reveals biological pathways associated with fetal programming in beef cattle.Scientific reports · 2024Article
- Stool and blood metabolomics in the metabolic syndrome: a cross-sectional study.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Serum metabolite signatures of cardiac function and morphology in individuals from a population-based cohort.Biomarker research · 2024Article
- Metabolomic associations of impaired awareness of hypoglycaemia in type 1 diabetes.Scientific reports · 2024Article
- Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming.Metabolites · 2023Article
- Circulating metabolites improve the prediction of renal impairment in patients with type 2 diabetes.BMJ open diabetes research & care · 2023Article
- Energy Metabolism Is Altered in Radioresistant Rectal Cancer.International journal of molecular sciences · 2023Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionGlobal metabolomics analyses using body fluids provide valuable results for the understanding and prediction of diseases. However, the mechanism of a disease is often tissue-based and it is advantageous to analyze metabolomic changes directly in the tissue. Metabolomics from tissue samples faces many challenges like tissue collection, homogenization, and metabolite extraction.
objectivesWe aimed to establish a metabolite extraction protocol optimized for tissue metabolite quantification by the targeted metabolomics Absolute
methodsWe quantified metabolites in samples of eleven murine tissues after extraction with three solvents (methanol, phosphate buffer, ethanol/phosphate buffer mixture) in two tissue to solvent ratios and analyzed the extraction yield, ionization efficiency, and reproducibility.
resultsWe found methanol and ethanol/phosphate buffer to be superior to phosphate buffer in regard to extraction yield, reproducibility, and ionization efficiency for all metabolites measured. Phosphate buffer, however, outperformed both organic solvents for amino acids and biogenic amines but yielded unsatisfactory results for lipids. The observed matrix effects of tissue extracts were smaller or in a similar range compared to those of human plasma.
conclusionWe provide for each murine tissue type an optimized high-throughput metabolite extraction protocol, which yields the best results for extraction, reproducibility, and quantification of metabolites in the p180 kit. Although the performance of the extraction protocol was monitored by the p180 kit, the protocol can be applicable to other targeted metabolomics assays.
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