ArticlePlant methods2018
High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 79 citations in OpenAlex.
- The development of a silage-based biorefinery to deliver the maximum nutritional benefit for human consumption from UK grasslands.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Amino Acid Analysis and Cytotoxicity Study of IraqiMolecules (Basel, Switzerland) · 2025Article
- Green Extraction Method: Microwave-Assisted Water Extraction Followed by HILIC-HRMS Analysis to Quantify Hydrophilic Compounds in Plants.Metabolites · 2025Article
- Impact of Packaging Variations on the Amino Acid Profile, Proximate Content, and Antinutritional Components of Tempeh from Jack Bean Sprouts.Preventive nutrition and food science · 2025Article
- Cultivar selection and processing methods are crucial tools for tailoring the physico-chemical properties and functionality of pea (Current research in food science · 2025Article
- Branched Chain Amino Acid Content and Antioxidant Activity of Mung Bean Tempeh Powder for Developing Oral Nutrition Supplements.Foods (Basel, Switzerland) · 2023Article
- An investigation on glutathione derived from spinach and red cabbage leaves and their effects of adding to meat patties.Saudi journal of biological sciences · 2023Article
- High-Throughput Analysis of Amino Acids for Protein Quantification in Plant and Animal-Derived Samples Using High Resolution Mass Spectrometry.Molecules (Basel, Switzerland) · 2021Article
- Integrated Isoform Sequencing and Dynamic Transcriptome Analysis Reveals Diverse Transcripts Responsible for Low Temperature Stress at Anther Meiosis Stage in Rice.Frontiers in plant science · 2021Article
- Ion-Trap Mass Spectrometric Analysis of Bisphenol A Interactions With Titanium Dioxide Nanoparticles and Milk Proteins.Molecules (Basel, Switzerland) · 2020Article
- Determination of immunogenic proteins in biopharmaceuticals by UHPLC-MS amino acid analysis.BMC chemistry · 2019Article
- Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters.Frontiers in chemistry · 2019Article
- Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry.Plant methods · 2019Article
- Quantification of Glutamate and Aspartate by Ultra-High Performance Liquid Chromatography.Molecules (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of samples but the classical amino acid analysis is limited by the fact that it is very time consuming with typical chromatographic run times of 70 min or more.
resultsWe have here developed a high-throughput method for analysis of amino acid profiles in plant materials. The method combines classical protein hydrolysis and derivatization with fast separation by UHPLC and detection by a single quadrupole (QDa) mass spectrometer. The chromatographic run time is reduced to 10 min and the precision, accuracy and sensitivity of the method are in line with other recent methods utilizing advanced and more expensive mass spectrometers. The sensitivity of the method is at least a factor 10 better than that of methods relying on detection by fluorescence or UV. It is possible to downscale sample size to 20 mg without compromising reproducibility, which makes the method ideal for analysis of very small sample amounts.
conclusionThe developed method allows high-throughput analysis of amino acid profiles in plant materials. The analysis is robust and accurate as well as compatible with both free amino acids and protein hydrolysates. The QDa detector offers high sensitivity and accuracy, while at the same time being relatively simple to operate and cheap to purchase, thus significantly reducing the overall analytical costs compared to methods based on more advanced mass spectrometers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.