ArticleAnalytical biochemistry2018
Rapid detection of potyviruses from crude plant extracts.
Article in Analytical biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 105 citations in OpenAlex.
- Development of rapid and visual Erwinia amylovora detection technology using recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD).Pest management science · 2026Article
- Field-parallel six-sample microfluidic detection of plant viruses via raffinose-assisted one-pot LAMP-CRISPR/Cas12b.Journal of advanced research · 2026Article
- Detection of TuMV by a toehold switch sensor coupled with NASBA amplification in Pseudostellaria heterophylla.Plant methods · 2025Article
- Development of reverse transcription recombinase polymerase amplification assay for rapid diagnostics of Peanut mottle virus.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Rapid detection of Impatiens necrotic spot virus from thrips vectors using reverse transcription-recombinase polymerase amplification.Scientific reports · 2024Article
- A novel system with robust compatibility and stability for detectingMicrobiology spectrum · 2024Article
- Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems.3 Biotech · 2024Review
- Development of reverse transcriptase-recombinase polymerase amplification (RT-RPA) assay for rapid detection of large cardamom chirke virus.Virusdisease · 2024Article
- Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses.Scientific reports · 2024Article
- Comparative Evaluation of PCR-Based, LAMP and RPA-CRISPR/Cas12a Assays for the Rapid Detection ofInternational journal of molecular sciences · 2024Article
- Simultaneous detection of bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) using recombinase polymerase amplification.Scientific reports · 2024Article
- Identification of yam mosaic virus as the main cause of yam mosaic diseases in Ethiopia.Heliyon · 2024Article
- A reverse transcription loop-mediated isothermal amplification assay for quick detection of tomato mosaic virus.PloS one · 2024Article
- Development of a multiplex RT-RPA assay for simultaneous detection of three viruses in cucurbits.Molecular plant pathology · 2023Article
- Article
- Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the Rapid and Sensitive Detection ofViruses · 2023Article
- The engineered CRISPR-Mb2Cas12a variant enables sensitive and fast nucleic acid-based pathogens diagnostics in the field.Plant biotechnology journal · 2023Article
- Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay.Frontiers in plant science · 2023Article
- Onsite detection of plant viruses using isothermal amplification assays.Plant biotechnology journal · 2022Review
- Viruses of Yams (Viruses · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of polysaccharides and polyphenols such as yam (Dioscorea spp.). Here, we report the development of a reliable, rapid and cost-effective detection method for the two most important potyviruses infecting yam based on reverse transcription-recombinase polymerase amplification (RT-RPA). The developed method, named 'Direct RT-RPA', detects each target virus directly from plant leaf extracts prepared with a simple and inexpensive extraction method avoiding laborious extraction of high-quality RNA. Direct RT-RPA enables the detection of virus-positive samples in under 30 min at a single low operation temperature (37 °C) without the need for any expensive instrumentation. The Direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories and seed certification facilities worldwide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.