Evidence mapPaperPMID 29396513Full record

ArticleScientific reports2018

PCSK9 induces a pro-inflammatory response in macrophages.

Chiara Ricci, Massimiliano Ruscica, Marina Camera, Laura Rossetti, Chiara Macchi, Alessandra Colciago, Ilaria Zanotti, Maria Giovanna Lupo, Maria Pia Adorni, Arrigo F G Cicero and 3 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
126citing papers in PubMed, 4 pooled it
24.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

126 citing papers in PubMed, 4 syntheses or guidelines pooled it, 216 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Guideline
  5. PCSK9 Inhibition During the Inflammatory Stage of SARS-CoV-2 Infection.Journal of the American College of Cardiology · 2023
    Trial
  6. Review
  7. Review
  8. Review
  9. Review
  10. PCSK9 in critical illness - It's not all about lipids.Annals of clinical biochemistry · 2026
    Review
  11. Review
  12. Article
  13. Exploration (Beijing, China) · 2026
    Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article

66 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Chiara RicciDipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.ORCID http://orcid.org/0000-0002-5148-8857
Massimiliano RuscicaDipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.ORCID http://orcid.org/0000-0002-0195-7061
Marina CameraDipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.
Laura RossettiCentro Cardiologico Monzino, Milan, Italy.
Chiara MacchiDipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.
Alessandra ColciagoDipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.
Ilaria ZanottiDipartimento di Scienze degli Alimenti e del Farmaco, Università di Parma, Parma, Italy.
Maria Giovanna LupoDipartimento di Scienze del Farmaco, Università degli Studi di Padova, Padua, Italy.ORCID http://orcid.org/0000-0002-4236-4414
Maria Pia AdorniDipartimento di Scienze degli Alimenti e del Farmaco, Università di Parma, Parma, Italy.
Arrigo F G CiceroDipartimento di Scienze Mediche e Chirurgiche, Università di Bologna, Bologna, Italy.
Federica FogacciDipartimento di Scienze Mediche e Chirurgiche, Università di Bologna, Bologna, Italy.ORCID http://orcid.org/0000-0001-7853-0042
Alberto CorsiniDipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.
Nicola FerriDipartimento di Scienze del Farmaco, Università degli Studi di Padova, Padua, Italy. nicola.ferri@unipd.it.
University of Milan · ITUniversity of Bologna · ITUniversity of Padua · ITUniversity of Parma · ITCentro Cardiologico Monzino · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intraplaque release of inflammatory cytokines from macrophages is implicated in atherogenesis by inducing the proliferation and migration of media smooth muscle cells (SMCs). PCSK9 is present and released by SMCs within the atherosclerotic plaque but its function is still unknown. In the present study, we tested the hypothesis that PCSK9 could elicit a pro-inflammatory effect on macrophages. THP-1-derived macrophages and human primary macrophages were exposed to different concentrations (0.250 ÷ 2.5 µg/ml) of human recombinant PCSK9 (hPCSK9). After 24 h incubation with 2.5 µg/ml PCSK9, a significant induction of IL-1β, IL-6, TNF-α, CXCL2, and MCP1 mRNA, were observed in both cell types. Co-culture of THP-1 macrophages with HepG2 overexpressing hPCSK9 also showed the induction of TNF-α (2.4 ± 0.5 fold) and IL-1β (8.6 ± 1.8 fold) mRNA in macrophages. The effect of hPCSK9 on TNF-α mRNA in murine LDLR

Indexed as

AnimalsCells, CulturedCoculture TechniquesCytokinesFemaleHumansMacrophagesMaleMiceProprotein Convertase 9CytokinesPCSK9 protein, humanPcsk9 protein, mouseProprotein Convertase 9

Identifiers

PMID29396513
PMCPMC5797178
OpenAlexW2807631284

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.