ArticlePLoS neglected tropical diseases2018
Transcriptomes of Trypanosoma brucei rhodesiense from sleeping sickness patients, rodents and culture: Effects of strain, growth conditions and RNA preparation methods.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Trypanosoma brucei cattle infections contain cryptic transmission-adapted bloodstream forms at low parasitaemia.Nature communications · 2025Article
- Transcriptome profiles of Trypanosoma brucei rhodesiense in Malawi reveal focus specific gene expression profiles associated with pathology.PLoS neglected tropical diseases · 2024Article
- Article
- Several different sequences are implicated in bloodstream-form-specific gene expression in Trypanosoma brucei.PLoS neglected tropical diseases · 2022Article
- LncRNAs Are Differentially Expressed between Wildtype and Cell Line Strains of African Trypanosomes.Non-coding RNA · 2022Article
- In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.PLoS neglected tropical diseases · 2021Article
- Insights into the functions and RNA binding ofParasitology · 2021Article
- Workflow ofInternational journal for parasitology. Parasites and wildlife · 2021Article
- The Occurrence of Malignancy inFrontiers in microbiology · 2021Article
- Roles of the Pumilio domain protein PUF3 inParasitology · 2020Article
- Blood signatures for second stage human African trypanosomiasis: a transcriptomic approach.BMC medical genomics · 2020Article
- The endoplasmic reticulum-associated mRNA-binding proteins ERBP1 and ERBP2 interact in bloodstream-formPeerJ · 2020Article
- High-Throughput Sequencing for Trypanosome Transcriptome Characterization.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- A gene expression comparison of Trypanosoma brucei and Trypanosoma congolense in the bloodstream of the mammalian host reveals species-specific adaptations to density-dependent development.PLoS neglected tropical diseases · 2018Article
- Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase.The Journal of biological chemistryArticle
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Authors and funding
11 authors.
Funding
Abstract
All of our current knowledge of African trypanosome metabolism is based on results from trypanosomes grown in culture or in rodents. Drugs against sleeping sickness must however treat trypanosomes in humans. We here compare the transcriptomes of Trypanosoma brucei rhodesiense from the blood and cerebrospinal fluid of human patients with those of trypanosomes from culture and rodents. The data were aligned and analysed using new user-friendly applications designed for Kinetoplastid RNA-Seq data. The transcriptomes of trypanosomes from human blood and cerebrospinal fluid did not predict major metabolic differences that might affect drug susceptibility. Usefully, there were relatively few differences between the transcriptomes of trypanosomes from patients and those of similar trypanosomes grown in rats. Transcriptomes of monomorphic laboratory-adapted parasites grown in in vitro culture closely resembled those of the human parasites, but some differences were seen. In poly(A)-selected mRNA transcriptomes, mRNAs encoding some protein kinases and RNA-binding proteins were under-represented relative to mRNA that had not been poly(A) selected; further investigation revealed that the selection tends to result in loss of longer mRNAs.
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