ArticleProtoplasma2018
Overexpression of tryptophan decarboxylase and strictosidine synthase enhanced terpenoid indole alkaloid pathway activity and antineoplastic vinblastine biosynthesis in Catharanthus roseus.
Article in Protoplasma, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Medicinal-plant immunity and specialized metabolism in plant-pathogen interactions: mechanisms and applications.Planta · 2026Review
- Novel indole alkaloids from the flower buds ofJournal of ginseng research · 2026Article
- Genome-scale metabolic model guided metabolic flux analysis in the endophyte Alternaria burnsii NCIM1409.Bioprocess and biosystems engineering · 2026Article
- Unlocking the potential of endophytes in enhancing plant secondary metabolite biosynthesis.Biochemistry and biophysics reports · 2026Review
- Enhanced accumulation of anticancer compounds in C. roseus hairy root cultures through elicitation and precursor feeding.Scientific reports · 2026Article
- Genome-wide association studies reveal genetic variants associated with antineoplastic monoterpenoid indole alkaloid accumulation inFrontiers in plant science · 2026Article
- Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis.Plants (Basel, Switzerland) · 2025Review
- Identification of DELLA and GID1 genes in Catharanthus roseus and their potential role in regulating vindoline biosynthesis.Plant molecular biology · 2025Article
- Sustainable vinblastine production by3 Biotech · 2025Article
- Molecular validation of genetically transformedHeliyon · 2024Article
- Efficient hairy root induction system of Astragalus membranaceus and significant enhancement of astragalosides via overexpressing AmUGT15.Plant cell reports · 2024Article
- Characterization of the ZCTs, a subgroup of Cys2-His2 zinc finger transcription factors regulating alkaloid biosynthesis in Catharanthus roseus.Plant cell reports · 2024Article
- Critical parameters for robustPlant direct · 2024Article
- Strategies on biosynthesis and production of bioactive compounds in medicinal plants.Chinese herbal medicines · 2024Review
- Omics analyses of Rehmannia glutinosa dedifferentiated and cambial meristematic cells reveal mechanisms of catalpol and indole alkaloid biosynthesis.BMC plant biology · 2023Article
- Cell type matters: competence for alkaloid metabolism differs in two seed-derived cell strains of Catharanthus roseus.Protoplasma · 2023Article
- Growth light substantially affects both primary and secondary metabolic processes inPhotosynthetica · 2023Article
- Ethyl Methane Sulfonate and Sodium Azide-Mediated Chemical and X-ray-Mediated Physical Mutagenesis Positively RegulatePlants (Basel, Switzerland) · 2022Article
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- Enhancement of Vindoline and Catharanthine Accumulation, Antioxidant Enzymes Activities, and Gene Expression Levels inMarine drugs · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Terpenoid indole alkaloid (TIA) biosynthetic pathway of Catharanthus roseus possesses the major attention in current metabolic engineering efforts being the sole source of highly expensive antineoplastic molecules vinblastine and vincristine. The entire TIA pathway is fairly known at biochemical and genetic levels except the pathway steps leading to biosynthesis of catharanthine and tabersonine. To increase the in-planta yield of these antineoplastic metabolites for the pharmaceutical and drug industry, extensive plant tissue culture-based studies were performed to provide alternative production systems. However, the strict spatiotemporal developmental regulation of TIA biosynthesis has restricted the utility of these cultures for large-scale production. Therefore, the present study was performed to enhance the metabolic flux of TIA pathway towards the biosynthesis of vinblastine by overexpressing two upstream TIA pathway genes, tryptophan decarboxylase (CrTDC) and strictosidine synthase (CrSTR), at whole plant levels in C. roseus. Whole plant transgenic of C. roseus was developed using Agrobacterium tumefaciens LBA1119 strain having CrTDC and CrSTR gene cassette. Developed transgenic lines demonstrated up to twofold enhanced total alkaloid production with maximum ninefold increase in vindoline and catharanthine, and fivefold increased vinblastine production. These lines recorded a maximum of 38-fold and 65-fold enhanced transcript levels of CrTDC and CrSTR genes, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.