Evidence map›Paper›PMID 29540444›Full record

ArticleG3 (Bethesda, Md.)2018

A Flow Cytometry-Based Phenotypic Screen To Identify Novel Endocytic Factors in

Kristie Wrasman, Salvatore L Alioto, Yorke Zhang, Kyle Hoban, Marjon Khairy, Bruce L Goode, Beverly Wendland

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kristie WrasmanDepartment of Biology, Johns Hopkins University Baltimore, MD 21218.
Salvatore L AliotoDepartment of Biology, Brandeis University, Waltham, MA 02453.
Yorke ZhangDepartment of Biology, Johns Hopkins University Baltimore, MD 21218.
Kyle HobanDepartment of Biology, Johns Hopkins University Baltimore, MD 21218.
Marjon KhairyDepartment of Biology, Johns Hopkins University Baltimore, MD 21218.
Bruce L GoodeDepartment of Biology, Brandeis University, Waltham, MA 02453.
Beverly WendlandDepartment of Biology, Johns Hopkins University Baltimore, MD 21218 bwendland@jhu.edu.

Funding

CELLULAR AND MOLECULAR BIOLOGYT32GM007231 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI VAN DOREN, MARK B · 1985 to 2020
$18.4M
MECHNISMS REGULATING ACTIN ASSEMBLYR01GM063691 · NIGMS · BRANDEIS UNIVERSITY · PI GOODE, BRUCE L · 2002 to 2019
$7.5M
MOLECULAR ANALYSIS OF ENDOCYTOSIS PROTEINS IN YEASTR01GM060979 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI WENDLAND, BEVERLY R · 2000 to 2013
$4.3M
NIGMS NIH HHS R01 GM060979NIGMS NIH HHS R01 GM063691NIGMS NIH HHS T32 GM007231
6 · The paper itself

Abstract

Endocytosis is a fundamental process for internalizing material from the plasma membrane, including many transmembrane proteins that are selectively internalized depending on environmental conditions. In most cells, the main route of entry is clathrin-mediated endocytosis (CME), a process that involves the coordinated activity of over 60 proteins; however, there are likely as-yet unidentified proteins involved in cargo selection and/or regulation of endocytosis. We performed a mutagenic screen to identify novel endocytic genes in

Indexed as

EndocytosisAmino Acid SequenceCell MembraneFlow CytometryGreen Fluorescent ProteinsMutagenesisMutation, MissensePhenotypePoint MutationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsGreen Fluorescent ProteinsPHluorinSaccharomyces cerevisiae ProteinsactinArp2/3cargo sortingclathrin-mediated endocytosisforward genetic screen

Identifiers

PMID29540444
PMCPMC5940143

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.