Evidence map›Paper›PMID 29556564›Full record

ArticleBiochemistry and biophysics reports2018

The role of the ShcD and RET interaction in neuroblastoma survival and migration.

Zeanap A Mabruk, Samrein B M Ahmed, Asha Caroline Thomas, Sally A Prigent

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Zeanap A MabrukSharjah Institute for Medical Research and College of Medicine University of Sharjah, United Arab Emirates.
Samrein B M AhmedSharjah Institute for Medical Research and College of Medicine University of Sharjah, United Arab Emirates.
Asha Caroline ThomasSharjah Institute for Medical Research and College of Medicine University of Sharjah, United Arab Emirates.
Sally A PrigentDepartment of Molecular and Cellular Biology, University of Leicester, UK.
University of Sharjah · AEUniversity of Leicester · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Preliminary screening data showed that the ShcD adaptor protein associates with the proto-oncogene RET receptor tyrosine kinase. In the present study, we aimed to investigate the molecular interaction between ShcD and RET in human neuroblastoma cells and study the functional impact of this interaction. We were able to show that ShcD immunoprecipitated with RET from SK-N-AS neuroblastoma cell lysates upon GDNF treatment. This result was validated by ShcD-RET co-localization, which was visualized using a fluorescence microscope. ShcD-RET coexpression promoted ShcD and RET endosomal localization, resulting in unexpected inhibition of the downstream ERK and AKT pathways. Interestingly, ShcD-RET association reduced the viability and migration of SK-N-AS cells. Although ShcD was previously shown to trigger melanoma cell migration and tumorigenesis, our data showed an opposite role for ShcD in neuroblastoma SK-N-AS cells via its association with RET in GDNF-treated cells. In conclusion, ShcD acts as a switch molecule that promotes contrasting biological responses depending on the stimulus ad cell type.

Indexed as

Akt,, Protein kinase B;ALK,, Anaplastic Lymphoma KinaseCMV,, CytomegalovirusDMEM,, Dulbecoo Modified Eagle's Medium;DNA,, Deoxyribonucleic AcidECL,, Enhanced Chemiluminescence;EGF,, Epidermal Growth Factor;EGFR,, Epidermal Growth Factor Receptor;EndosomesERK,, Extracellular Signal–Regulated Kinases;FBS,, Fetal Bovine SerumFGFR,, fibroblast growth factor receptorsGDNFGDNF,, Glial Cell Line-Derived Neurotropic Factor;GFLs,, GDNF Family Ligands;GFP,, Green Fluorescent ProteinGPCR,, G-Protein Coupled ReceptorGRB2,, Growth Factor Receptor-Bound Protein 2;HGFR,, hepatocyte growth factor receptor;HRP,, Horseradish Peroxidasehrs,, HoursIGF,, Insulin Growth Factor;LB,, Luria-BertanimAb,, Monoclonal AntibodyMAPK,, Mitogen-Activated Protein KinasesMAP,, Mitogen-Activated Protein;min,, MinuteMuSK,, Muscle Specific KinaseNeuroblastomaNFDM,, Non-Fat Dry MilkpAb,, Polyclonal AntibodyPBS,, Phosphate-Buffered SalinePBST,, Phosphate-Buffered Saline TweenPDGF,, Platelet-Derived Growth Factor;PI3K,, Phosphoinositide 3-KinasePMSF,, Phenylmethylsulfonyl FluoridepTyr,, Phospho-TyrosinePVDF,, Polyvinylidene FluorideRETRET,, Rearranged During Transfectionrpm,, revolution per minute;RTKs,, Receptor Tyrosine KinaseRT,, Room Temperature;SDS-PAGE,, Sodium Dodecylsulphate Polyacrylamide Gel ElectrophoresisShcDShcD,, Src Homology And Collagen DSrc,, Proto-Oncogene Tyrosine-Protein Kinase SrcTKRs,, Tyrosine Kinase Receptor;TrkA/B/C,, Tropomyosin-Related Kinase Receptor A/B/C

Identifiers

PMID29556564
PMCPMC5857170
OpenAlexW2791690542

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.