ArticleBioelectrochemistry (Amsterdam, Netherlands)2018
The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency.
Article in Bioelectrochemistry (Amsterdam, Netherlands), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Minimization of Cancellation Effect with Nisin During Bipolar Nanosecond Electrochemotherapy.International journal of molecular sciences · 2026Article
- NaThe Journal of membrane biology · 2026Article
- Efficacy of Plasmid DNA Delivery into Mice by Intradermal Injections Alone and Facilitated by Sonoporation or Electroporation.Vaccines · 2026Article
- Peptide-Assisted Membrane Permeabilization: Effects of Waveform Polarity, Inter-Monopulse Interval, and Surface Charge in Red Blood Cells and Planar Lipid Bilayers.The Journal of membrane biology · 2026Article
- Evaluation of unipolar and bipolar nanosecond pulses for calcium electrochemotherapy and immune response.Frontiers in immunology · 2026Article
- Nanosecond Pulsed Electric Fields (nsPEFs) for Precision Intracellular Oncotherapy: Recent Advances and Emerging Directions.International journal of molecular sciences · 2025Review
- The Effects of Bipolar Cancellation Phenomenon on Nano-Electrochemotherapy of Melanoma Tumors: In Vitro and In Vivo Pilot.International journal of molecular sciences · 2024Article
- Article
- Calcium Electrochemotherapy for Tumor Eradication and the Potential of High-Frequency Nanosecond Protocols.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction.International journal of molecular sciences · 2023Article
- Action spectra and mechanisms of (in) efficiency of bipolar electric pulses at electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Does the shape of the electric pulse matter in electroporation?Frontiers in oncology · 2022Review
- Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells.International journal of molecular sciences · 2021Article
- Electrotherapies for Glioblastoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Peculiarities of Neurostimulation by Intense Nanosecond Pulsed Electric Fields: How to Avoid Firing in Peripheral Nerve Fibers.International journal of molecular sciences · 2021Article
- 2-ns Electrostimulation of CaBiophysical journal · 2021Article
- Quadrupoles for Remote Electrostimulation Incorporating Bipolar Cancellation.Bioelectricity · 2020Article
- Dye Transport through Bilayers Agrees with Lipid Electropore Molecular Dynamics.Biophysical journal · 2020Article
- Real-Time Impedance Monitoring During Electroporation Processes in Vegetal Tissue Using a High-Performance Generator.Sensors (Basel, Switzerland) · 2020Article
- Article
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6 authors.
Funding
Abstract
Bipolar cancellation refers to a phenomenon when applying a second electric pulse reduces ("cancels") cell membrane damage by a preceding electric pulse of the opposite polarity. Bipolar cancellation is a reason why bipolar nanosecond electric pulses (nsEP) cause weaker electroporation than just a single unipolar phase of the same pulse. This study was undertaken to explore the dependence of bipolar cancellation on nsEP parameters, with emphasis on the amplitude ratio of two opposite polarity phases of a bipolar pulse. Individual cells (CHO, U937, or adult mouse ventricular cardiomyocytes (VCM)) were exposed to either uni- or bipolar trapezoidal nsEP, or to nanosecond electric field oscillations (NEFO). The membrane injury was evaluated by time-lapse confocal imaging of the uptake of propidium (Pr) or YO-PRO-1 (YP) dyes and by phosphatidylserine (PS) externalization. Within studied limits, bipolar cancellation showed little or no dependence on the electric field intensity, pulse repetition rate, chosen endpoint, or cell type. However, cancellation could increase for larger pulse numbers and/or for longer pulses. The sole most critical parameter which determines bipolar cancellation was the phase ratio: maximum cancellation was observed with the 2nd phase of about 50% of the first one, whereas a larger 2nd phase could add a damaging effect of its own. "Swapping" the two phases, i.e., delivering the smaller phase before the larger one, reduced or eliminated cancellation. These findings are discussed in the context of hypothetical mechanisms of bipolar cancellation and electroporation by nsEP.
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