Evidence map›Paper›PMID 29670029›Full record

ReviewViruses2018

Concepts in Light Microscopy of Viruses.

Robert Witte, Vardan Andriasyan, Fanny Georgi, Artur Yakimovich, Urs F Greber

Open access · goldAbstract readReview
In one paragraph

Review in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
7.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Label-free microscopy for virus infections.Microscopy (Oxford, England) · 2023
    Article
  11. Review
  12. Review
  13. Review
  14. Virus morphology: Insights from super-resolution fluorescence microscopy.Biochimica et biophysica acta. Molecular basis of disease · 2022
    Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Robert WitteDepartment of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. robert.witte@uzh.ch.
Vardan AndriasyanDepartment of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. vardan.andriasyan@uzh.ch.ORCID 0000-0002-9619-6655
Fanny GeorgiDepartment of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. fanny.georgi@uzh.ch.
Artur YakimovichMRC Laboratory for Molecular Cell Biology, University College London, Gower St., London WC1E 6BT, UK. a.yakimovich@ucl.ac.uk.ORCID 0000-0003-2458-4904
Urs F GreberDepartment of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. urs.greber@imls.uzh.ch.ORCID 0000-0003-2278-120X
University of Zurich · CHMRC Laboratory for Molecular Cell Biology · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viruses threaten humans, livestock, and plants, and are difficult to combat. Imaging of viruses by light microscopy is key to uncover the nature of known and emerging viruses in the quest for finding new ways to treat viral disease and deepening the understanding of virus–host interactions. Here, we provide an overview of recent technology for imaging cells and viruses by light microscopy, in particular fluorescence microscopy in static and live-cell modes. The review lays out guidelines for how novel fluorescent chemical probes and proteins can be used in light microscopy to illuminate cells, and how they can be used to study virus infections. We discuss advantages and opportunities of confocal and multi-photon microscopy, selective plane illumination microscopy, and super-resolution microscopy. We emphasize the prevalent concepts in image processing and data analyses, and provide an outlook into label-free digital holographic microscopy for virus research.

Indexed as

AnimalsHost-Pathogen InteractionsHumansImage Processing, Computer-AssistedMicroscopy, FluorescenceOptical ImagingPlantsVirusesadeno-associated virus AAVadenovirusbaculoviruscell biologycomputingcytoskeletondata analysisDNA virusendocytosisenveloped virusfluorescence microscopyfluorescent virionsgene expressiongene therapyhepatitis B virusherpes simplex virusherpesvirushigh-throughput screening, modelinghuman immunodeficiency virus HIVimage analysisimmunofluorescence microscopyinfectioninfluenza virusinnate immunityinternalizationintracellular transportlight microscopylive imagingmachine learningmembrane trafficmicroscopynonenveloped virusparvovirusquantitative microscopyreceptorRNA virussimian virus 40simulationsuper-resolutiontraffickingvirion uncoatingvirus entryvirus infectionvirus labeling

Identifiers

PMID29670029
PMCPMC5923496
OpenAlexW2799673346

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.