ArticlePloS one2018
IFN-λ and microRNAs are important modulators of the pulmonary innate immune response against influenza A (H1N2) infection in pigs.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Identification of trait-associated microRNA modules in liver transcriptome of pig fed with PUFAs-enriched supplementary diet.Journal of applied genetics · 2025Article
- Enteral plasma feeding improves gut function and immunity in piglets after birth asphyxia.Pediatric research · 2025Article
- The forecasting power of the mucin-microbiome interplay in livestock respiratory diseases.The veterinary quarterly · 2024Article
- LINC01686 affects LPS-induced cytokine expression via the miR-18a-5p/A20/STAT1 axis in THP-1 cells.Immunity, inflammation and disease · 2024Article
- MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges.Frontiers in veterinary science · 2024Review
- Prognostic significance of serum miR-18a-5p in severe COVID-19 Egyptian patients.Journal, genetic engineering & biotechnology · 2023Article
- Agaricus subrufescens fermented rye affects the development of intestinal microbiota, local intestinal and innate immunity in suckling-to-nursery pigs.Animal microbiome · 2023Article
- Decreased water temperature enhanceFrontiers in veterinary science · 2023Article
- Innate antiviral responses in porcine nasal mucosal explants inoculated with influenza A virus are comparable with responses in respiratory tissues after viral infection.Immunobiology · 2022Article
- miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.International journal of molecular sciences · 2021Review
- Differential miRNA Expression in Human Macrophage-Like Cells Infected withJournal of fungi (Basel, Switzerland) · 2021Article
- The Importance of miRNA Identification During Respiratory Viral Infections.Journal of cellular immunology · 2021Article
- The effects of the Xijiao Dihuang decoction combined with Yinqiao powder on miRNA-mRNA profiles in mice infected with influenza a virus.BMC complementary medicine and therapies · 2020Article
- Upregulation of miR-101 during Influenza A Virus Infection Abrogates Viral Life Cycle by Targeting mTOR Pathway.Viruses · 2020Article
- MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock.Frontiers in veterinary science · 2020Review
- Porcine Interferon Complex and Co-Evolution with Increasing Viral Pressure after Domestication.Viruses · 2019Review
- Reduced peripheral blood miR-140 may be a biomarker for acute lung injury by targeting Toll-like receptor 4 (TLR4).Experimental and therapeutic medicine · 2018Article
- LncRNA CASC2 is involved in the development of chronic obstructive pulmonary diseaseTherapeutic advances in respiratory diseaseArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The innate immune system is paramount in the response to and clearance of influenza A virus (IAV) infection in non-immune individuals. Known factors include type I and III interferons and antiviral pathogen recognition receptors, and the cascades of antiviral and pro- and anti-inflammatory gene expression they induce. MicroRNAs (miRNAs) are increasingly recognized to participate in post-transcriptional modulation of these responses, but the temporal dynamics of how these players of the antiviral innate immune response collaborate to combat infection remain poorly characterized. We quantified the expression of miRNAs and protein coding genes in the lungs of pigs 1, 3, and 14 days after challenge with swine IAV (H1N2). Through RT-qPCR we observed a 400-fold relative increase in IFN-λ3 gene expression on day 1 after challenge, and a strong interferon-mediated antiviral response was observed on days 1 and 3 accompanied by up-regulation of genes related to the pro-inflammatory response and apoptosis. Using small RNA sequencing and qPCR validation we found 27 miRNAs that were differentially expressed after challenge, with the highest number of regulated miRNAs observed on day 3. In contrast, the number of protein coding genes found to be regulated due to IAV infection peaked on day 1. Pulmonary miRNAs may thus be aimed at fine-tuning the initial rapid inflammatory response after IAV infection. Specifically, we found five miRNAs (ssc-miR-15a, ssc-miR-18a, ssc-miR-21, ssc-miR-29b, and hsa-miR-590-3p)-four known porcine miRNAs and one novel porcine miRNA candidate-to be potential modulators of viral pathogen recognition and apoptosis. A total of 11 miRNAs remained differentially expressed 14 days after challenge, at which point the infection had cleared. In conclusion, the results suggested a role for miRNAs both during acute infection as well as later, with the potential to influence lung homeostasis and susceptibility to secondary infections in the lungs of pigs after IAV infection.
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