Evidence map›Paper›PMID 29738431›Full record

ArticleViruses2018

A VP26-mNeonGreen Capsid Fusion HSV-2 Mutant Reactivates from Viral Latency in the Guinea Pig Genital Model with Normal Kinetics.

Julianna R Pieknik, Andrea S Bertke, Shuang Tang, Philip R Krause

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Julianna R PieknikUniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. julianna.pieknik@fda.hhs.gov.
Andrea S BertkeDepartment of Population Health Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. asbertke@vt.edu.ORCID 0000-0002-8941-8010
Shuang TangCenter for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA. Shuang.Tang@fda.hhs.gov.
Philip R KrauseCenter for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA. Philip.Krause@fda.hhs.gov.ORCID 0000-0002-1045-7536
Center for Biologics Evaluation and Research · USVirginia Tech · US

Funding

Neuron Specific Regulation of HSV1 and HSV2 Outcomes of InfectionR01NS104351 · NINDS · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI BERTKE, ANDREA S · 2018 to 2022
$1.7M
The Role of the Autonomic Nervous System in HSV InfectionK22AI097299 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI BERTKE, ANDREA S · 2013 to 2014
$270k
NIAID NIH HHS K22 AI097299NINDS NIH HHS R01 NS104351
6 · The paper itself

Abstract

Fluorescent herpes simplex viruses (HSV) are invaluable tools for localizing virus in cells, permitting visualization of capsid trafficking and enhancing neuroanatomical research. Fluorescent viruses can also be used to study virus kinetics and reactivation in vivo. Such studies would be facilitated by fluorescent herpes simplex virus recombinants that exhibit wild-type kinetics of replication and reactivation and that are genetically stable. We engineered an HSV-2 strain expressing the fluorescent mNeonGreen protein as a fusion with the VP26 capsid protein. This virus has normal replication and in vivo recurrence phenotypes, providing an essential improved tool for further study of HSV-2 infection.

Indexed as

Virus LatencyAnimalsCapsidCapsid ProteinsChlorocebus aethiopsDisease Models, AnimalFemaleGreen Fluorescent ProteinsGuinea PigsHerpes GenitalisHerpesvirus 2, HumanPhenotypeProtein TransportRecombinant Fusion ProteinsVaginaVero CellsCapsid ProteinsGreen Fluorescent ProteinsRecombinant Fusion Proteinsfluorescent HSV-2guinea pigHSV-2mNeonGreenreactivationvaginal infection model

Identifiers

PMID29738431
PMCPMC5977239
OpenAlexW2801407203

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.