ArticleToxins2018
Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe.
Article in Toxins, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Molecular Docking Insight into the Label-Free Fluorescence Aptasensor for Ochratoxin A Detection.Molecules (Basel, Switzerland) · 2023Article
- A Label-Free Fluorescence Aptasensor Based on G-Quadruplex/Thioflavin T Complex for the Detection of Trypsin.Molecules (Basel, Switzerland) · 2022Article
- Novel DNA Aptameric Sensors to Detect the Toxic Insecticide Fenitrothion.International journal of molecular sciences · 2021Article
- Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJToxins · 2020Article
- Graphene/aptamer probes for small molecule detection: from in vitro test to in situ imaging.Mikrochimica acta · 2020Review
- Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection.Frontiers in chemistry · 2020Review
- Review
- Label-Free Fluorescent Aptasensor for Ochratoxin-A Detection Based on CdTe Quantum Dots and (Toxins · 2019Article
- A rapid fluorometric method for determination of aflatoxin BMikrochimica acta · 2019Article
- Fluorescent Method for the Detection of Biothiols Using an Ag⁺-Mediated Conformational Switch.Sensors (Basel, Switzerland) · 2019Article
- Article
- A Label-Free Fluorescent Assay for the Rapid and Sensitive Detection of Adenosine Deaminase Activity and Inhibition.Sensors (Basel, Switzerland) · 2018Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ochratoxin A (OTA) is one of the most common mycotoxins contaminating feed and foodstuffs. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, a fast and sensitive fluorescence aptamer biosensor has been proposed for the OTA assay. In the absence of OTA, the OTA aptamer can form a G-quadruplex structure with thioflavin T (ThT) dye, which results in increased fluorescence. After joining OTA, OTA aptamer combines with OTA and the G-quadruplex can be formed. Only faint fluorescence was finally observed when ThT weakly reacts with the quadruplex. Through this test method, the entire reaction and analysis process of OTA can be completed in 10 min. Under optimal experimental conditions (600 nM OTA-APT, 7 μM ThT, and 3 min incubation time), this proposed assay has a good limit of detection (LOD) of 0.4 ng/mL and shows a good linear relationship within the range of 1.2⁻200 ng/mL under the best experimental conditions. This method has a high specificity for OTA relative to Ochratoxin B (23%) and Aflatoxin B₁ (13%). In addition, the quantitative determination of this method in real samples has been validated using a sample of red wine supplemented with a range of OTA concentrations (1.2 ng/mL, 12 ng/mL, and 40 ng/mL) with recoveries of 96.5% to 107%.
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