ArticlePLoS biology2018
High-dimensional single-cell phenotyping reveals extensive haploinsufficiency.
Article in PLoS biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of dispensable essential genes and their interactions with bypass suppressors.Life science alliance · 2024Pooled it
- Depletion of aneuploid cells is shaped by cell-to-cell interactions.Cell genomics · 2025Article
- Effect of heterozygous deletions on phenotypic changes and dosage compensation in Arabidopsis thaliana.Scientific reports · 2025Article
- SSBD: an ecosystem for enhanced sharing and reuse of bioimaging data.Nucleic acids research · 2025Article
- Systematic data analysis pipeline for quantitative morphological cell phenotyping.Computational and structural biotechnology journal · 2024Review
- Article
- Rational selection of morphological phenotypic traits to extract essential similarities in chemical perturbation in the ergosterol pathway.Scientific reports · 2024Article
- Translation variation across genetic backgrounds reveals a post-transcriptional buffering signature in yeast.Nucleic acids research · 2024Article
- Application of unimodal probability distribution models for morphological phenotyping of budding yeast.FEMS yeast research · 2024Article
- Large-scale phenogenomic analysis of human cancers uncovers frequent alterations affecting SMC5/6 complex components in breast cancer.NAR cancer · 2023Article
- Targeted Mutations Produce Divergent Characteristics in Pedigreed Sake Yeast Strains.Microorganisms · 2023Article
- Targeted deletion of ecto-5'-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes).Scientific reports · 2022Article
- Quantitative Characterisation of Low Abundant Yeast Mitochondrial Proteins Reveals Compensation for Haplo-Insufficiency in Different Environments.International journal of molecular sciences · 2022Article
- Review
- Article
- Assignment of unimodal probability distribution models for quantitative morphological phenotyping.BMC biology · 2022Article
- High-throughput platform for yeast morphological profiling predicts the targets of bioactive compounds.NPJ systems biology and applications · 2022Article
- The good, the bad, and the ugly: Evolutionary and pathological aspects of gene dosage alterations.PLoS genetics · 2021Review
- Defining Functions of Mannoproteins inJournal of fungi (Basel, Switzerland) · 2021Article
- Polyploid engineering by increasing mutant gene dosage in yeasts.Microbial biotechnology · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haploinsufficiency, a dominant phenotype caused by a heterozygous loss-of-function mutation, has been rarely observed. However, high-dimensional single-cell phenotyping of yeast morphological characteristics revealed haploinsufficiency phenotypes for more than half of 1,112 essential genes under optimal growth conditions. Additionally, 40% of the essential genes with no obvious phenotype under optimal growth conditions displayed haploinsufficiency under severe growth conditions. Haploinsufficiency was detected more frequently in essential genes than in nonessential genes. Similar haploinsufficiency phenotypes were observed mostly in mutants with heterozygous deletion of functionally related genes, suggesting that haploinsufficiency phenotypes were caused by functional defects of the genes. A global view of the gene network was presented based on the similarities of the haploinsufficiency phenotypes. Our dataset contains rich information regarding essential gene functions, providing evidence that single-cell phenotyping is a powerful approach, even in the heterozygous condition, for analyzing complex biological systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.