Evidence map›Paper›PMID 29807339›Full record

ArticleColloids and surfaces. B, Biointerfaces2018

In vitro evaluation of lysophosphatidic acid delivery via reverse perfluorocarbon emulsions to enhance alveolar epithelial repair.

Diane L Nelson, Yutong Zhao, Mario L Fabiilli, Keith E Cook

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Colloids and surfaces. B, Biointerfaces, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Diane L NelsonDepartment of Biomedical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Scott Hall 4th Floor, Pittsburgh, PA, 15213, USA. Electronic address: dlnelson@andrew.cmu.edu.
Yutong ZhaoDepartment of Medicine, University of Pittsburgh, Division of Pulmonary, Allergy and Critical Care Medicine, East 1200A Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA, 15213, USA. Electronic address: zhaoy3@upmc.edu.
Mario L FabiilliDepartment of Radiology, University of Michigan, 3226A Medical Sciences Building I, 1301 Catherine Street, Ann Arbor, MI, 48109, USA. Electronic address: mfabiill@med.umich.edu.
Keith E CookDepartment of Biomedical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Scott Hall 4th Floor, Pittsburgh, PA, 15213, USA. Electronic address: keicook@andrew.cmu.edu.
Carnegie Mellon University · USUniversity of Michigan · USUniversity of Pittsburgh · US

Funding

Molecular regulation of anti-inflammatory cytokine receptor in sepsisR01HL136294 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ZHAO, YUTONG · 2018 to 2021
$1.8M
Regulation of proteolysis by deubiquiting enzyme in lung inflammatory diseaseR01HL131665 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ZHAO, YUTONG · 2017 to 2020
$1.5M
NHLBI NIH HHS R01 HL131665NHLBI NIH HHS R01 HL136294
6 · The paper itself

Abstract

backgroundAlveolar drug delivery is needed to enhance alveolar repair during acute respiratory distress syndrome. However, delivery of inhaled drugs is poor in this setting. Drug delivery via liquid perfluorocarbon emulsions could address this problem through better alveolar penetration and improved spatial distribution. Therefore, this study investigated the efficacy of the delivery of lysophosphatidic acid (LPA) growth factor to cultured alveolar epithelial cells via a perfluorocarbon emulsion.

methodsMurine alveolar epithelial cells were treated for 2 h with varying concentrations (0-10 μM) of LPA delivered via aqueous solution or PFC emulsion. Cell migration was evaluated 18 h post-treatment using a scratch assay. Barrier function was evaluated 1 h post-treatment using a permeability assay. Proliferation was evaluated 72 h post-treatment using a viability assay.

resultsPartially due to emulsion creaming and stability, the effects of LPA were either diminished or completely hindered when delivered via emulsion versus aqueous. Migration increased significantly following treatment with the 10 μM emulsion (p < 10

conclusionsThe availability and thus the biological effect of LPA is significantly blunted during emulsified delivery in vitro, and this attenuation depends on the specific cellular function examined. Thus, the cellular level effects of drug delivery to the lungs via PFC emulsion are likely to vary based on the drug and the effect it is intended to create.

Indexed as

Drug Delivery SystemsAlveolar Epithelial CellsAnimalsAnti-Bacterial AgentsCell MovementCell ProliferationCells, CulturedEmulsionsFluorocarbonsInflammationLysophospholipidsMiceParticle SizeSurface PropertiesAnti-Bacterial AgentsEmulsionsFluorocarbonslysophosphatidic acidLysophospholipidsAcute respiratory distress syndromeLysophosphatidic acidPartial liquid ventilationPerfluorocarbon

Identifiers

PMID29807339
PMCPMC6428640
OpenAlexW2804983671

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.