ArticleCurrent biology : CB2018
Stochastic Seeding Coupled with mRNA Self-Recruitment Generates Heterogeneous Drosophila Germ Granules.
Article in Current biology : CB, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 69 citations in OpenAlex.
- Conserved RNA helicase Vasa regulates ribonucleoprotein condensate dynamics and mRNA localization.iScience · 2026Article
- Discrete 3' UTR sequence motifs regulate nanos RNA enrichment in Drosophila germ granules.Developmental biology · 2026Article
- How do RNA molecules distinguish self from non-self?Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Conserved RNA helicase Vasa regulates ribonucleoprotein condensates through protein interaction and mRNA recruitment.bioRxiv : the preprint server for biology · 2025Article
- Multiplexed smFISH Reveals the Spatial Organization of Neuropil Localized mRNAs Is Linked to Abundance.eNeuro · 2025Article
- Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development.Nature communications · 2025Article
- Review
- The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules.PLoS genetics · 2025Article
- How germ granules promote germ cell fate.Nature reviews. Genetics · 2024Review
- Spatial organization of translation and translational repression in two phases of germ granules.Nature communications · 2024Article
- Direct observation of translational activation by a ribonucleoprotein granule.Nature cell biology · 2024Article
- Caenorhabditis elegans germ granules accumulate hundreds of low translation mRNAs with no systematic preference for germ cell fate regulators.Development (Cambridge, England) · 2024Article
- Smaug regulates germ plasm assembly and primordial germ cell number in Drosophila embryos.Science advances · 2024Article
- Phase Separation as a Driver of Stem Cell Organization and Function during Development.Journal of developmental biology · 2023Review
- Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1.Developmental cell · 2023Article
- Review
- Germ Granule Evolution Provides Mechanistic Insight into Drosophila Germline Development.Molecular biology and evolution · 2023Article
- Localization of oskar mRNA by agglomeration in ribonucleoprotein granules.PLoS genetics · 2023Article
- Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development.FEBS letters · 2023Article
- RNA granules: functional compartments or incidental condensates?Genes & development · 2023Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The formation of ribonucleoprotein assemblies called germ granules is a conserved feature of germline development. In Drosophila, germ granules form at the posterior of the oocyte in a specialized cytoplasm called the germ plasm, which specifies germline fate during embryogenesis. mRNAs, including nanos (nos) and polar granule component (pgc), that function in germline development are localized to the germ plasm through their incorporation into germ granules, which deliver them to the primordial germ cells. Germ granules are nucleated by Oskar (Osk) protein and contain varying combinations and quantities of their constituent mRNAs, which are organized as spatially distinct, multi-copy homotypic clusters. The process that gives rise to such heterogeneous yet organized granules remains unknown. Here, we show that individual nos and pgc transcripts can populate the same nascent granule, and these first transcripts then act as seeds, recruiting additional like transcripts to form homotypic clusters. Within a granule, homotypic clusters grow independently of each other but depend on the simultaneous acquisition of additional Osk. Although granules can contain multiple clusters of a particular mRNA, granule mRNA content is dominated by cluster size. These results suggest that the accumulation of mRNAs in the germ plasm is controlled by the mRNAs themselves through their ability to form homotypic clusters; thus, RNA self-association drives germ granule mRNA localization. We propose that a stochastic seeding and self-recruitment mechanism enables granules to simultaneously incorporate many different mRNAs while ensuring that each becomes enriched to a functional threshold.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.