Evidence map›Paper›PMID 29964320›Full record

ArticleEnvironmental toxicology2018

Alterations in cellular lipid metabolism produce neutral lipid accumulation following exposure to the organochlorine compound trans-nonachlor in rat primary hepatocytes.

George Eli Howell, Erin McDevitt, Lucie Henein, Charlee Mulligan, Darian Young

Abstract read
In one paragraph

Article in Environmental toxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

George Eli HowellDepartment of Basic Sciences, Mississippi State University College of Veterinary Medicine, Mississippi State, Mississippi.ORCID http://orcid.org/0000-0001-8761-976X
Erin McDevittDepartment of Basic Sciences, Mississippi State University College of Veterinary Medicine, Mississippi State, Mississippi.
Lucie HeneinDepartment of Basic Sciences, Mississippi State University College of Veterinary Medicine, Mississippi State, Mississippi.
Charlee MulliganDepartment of Basic Sciences, Mississippi State University College of Veterinary Medicine, Mississippi State, Mississippi.
Darian YoungDepartment of Basic Sciences, Mississippi State University College of Veterinary Medicine, Mississippi State, Mississippi.
Mississippi State University · US

Funding

Effects of organochlorine pesticide exposure on hepatic lipid metabolism in type 2 diabetesR15ES026791 · NIEHS · MISSISSIPPI STATE UNIVERSITY · PI HOWELL, GEORGE E · 2016 to 2016
$435k
NIEHS NIH HHS R15 ES026791
6 · The paper itself

Abstract

Recent epidemiological studies have revealed significant positive associations between exposure to organochlorine (OC) pesticides and occurrence of the metabolic syndrome and there are a growing number of animal-based studies to support causality. However, the cellular mechanisms linking OC compound exposure and metabolic dysfunction remain elusive. Therefore, the present study was designed to determine if direct exposure to three highly implicated OC compounds promoted hepatic steatosis, the hepatic ramification of the metabolic syndrome. First, the steatotic effect of p,p'-dichlorodiphenyldichloroethylene (DDE), oxychlordane, and trans-nonachlor was determined in freshly isolated rat primary hepatocytes. Exposure to trans-nonachlor significantly increased neutral lipid accumulation as opposed to DDE and oxychlordane. To determine possible mechanisms governing increased fatty acid availability, the effects of trans-nonachlor exposure on fatty acid uptake, de novo lipogenesis, triglyceride secretion, and fatty acid oxidation were explored. Trans-nonachlor did not significantly alter fatty acid uptake. However, insulin-stimulated de novo lipogenesis as well as basal expression of fatty acid synthase, a major regulator of lipogenesis were significantly increased following trans-nonachlor exposure. Interestingly, there was a significant decrease in fatty acid oxidation following trans-nonachlor exposure. This decrease in fatty acid oxidation was accompanied by a slight, but significant increase in oleic acid-induced cellular triglyceride secretion. Therefore, taken together, the present data indicate direct exposure to trans-nonachlor has a more potent pro-steatotic effect than exposure to DDE or oxychlordane. This pro-steatotic effect of trans-nonachlor appears to be predominately mediated via increased de novo lipogenesis and decreased fatty acid oxidation.

Indexed as

AnimalsChlordanDichlorodiphenyl DichloroethyleneFatty AcidsFatty Acid Synthase, Type IHepatocytesHydrocarbons, ChlorinatedInsulinLipid MetabolismLipogenesisMaleOxidation-ReductionPesticidesRats, Sprague-DawleyTriglyceridesChlordanDichlorodiphenyl DichloroethyleneFatty AcidsFatty Acid Synthase, Type IHydrocarbons, ChlorinatedInsulinnonachloroxychlordanePesticidesTriglyceridesde novo lipogenesisfatty acid oxidationhepatic steatosisprimary hepatocytetrans-nonachlor

Identifiers

PMID29964320
PMCPMC6105551
OpenAlexW2809738495

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.