Evidence mapPaperPMID 30016149Full record

ArticleAmerican journal of physiology. Endocrinology and metabolism2018

Diabetes-associated alterations in the cecal microbiome and metabolome are independent of diet or environment in the UC Davis Type 2 Diabetes Mellitus Rat model.

Brian D Piccolo, James L Graham, Kimber L Stanhope, Intawat Nookaew, Kelly E Mercer, Sree V Chintapalli, Umesh D Wankhade, Kartik Shankar, Peter J Havel, Sean H Adams

Abstract read
In one paragraph

Article in American journal of physiology. Endocrinology and metabolism, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. The Role of Precision Nutrition in Kidney Disease.Clinical journal of the American Society of Nephrology : CJASN · 2026
    Review
  2. Article
  3. Wild blueberries and cardiometabolic health: a current review of the evidence.Critical reviews in food science and nutrition · 2026
    Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Brian D PiccoloArkansas Children's Nutrition Center , Little Rock, Arkansas.
James L GrahamDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California , Davis, California.
Kimber L StanhopeDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California , Davis, California.
Intawat NookaewDepartment of Biomedical Informatics, University of Arkansas for Medical Sciences , Little Rock, Arkansas.
Kelly E MercerArkansas Children's Nutrition Center , Little Rock, Arkansas.
Sree V ChintapalliArkansas Children's Nutrition Center , Little Rock, Arkansas.
Umesh D WankhadeArkansas Children's Nutrition Center , Little Rock, Arkansas.
Kartik ShankarArkansas Children's Nutrition Center , Little Rock, Arkansas.
Peter J HavelDepartment of Molecular Biosciences, School of Veterinary Medicine, University of California , Davis, California.
Sean H AdamsArkansas Children's Nutrition Center , Little Rock, Arkansas.

Funding

The role of autophagy in bone remodelingP20GM125503 · NIGMS · UNIV OF ARKANSAS FOR MED SCIS · 2023 to 2025
$6.8M
Role of MED12L- Associated Chromatin Enhancers in Pediatric Acute Megakaryoblastic LeukemiaP20GM121293 · ARKANSAS CHILDREN'S HOSPITAL RES INST · 2025 to 2025
$2.3M
BICARBONATE TRANSPORT BY THE MATURING RENAL TUBULER01DK050603 · UNIVERSITY OF ROCHESTER · 1995 to 2003
$1.4M
NHLBI NIH HHS R01 HL091333NHLBI NIH HHS R01 HL107256NHLBI NIH HHS R01 HL121324NIDDK NIH HHS R01 DK050603NIDDK NIH HHS R01 DK078328NIDDK NIH HHS RC1 DK087307NIDDK NIH HHS U24 DK092993NIGMS NIH HHS P20 GM121293NIGMS NIH HHS P20 GM125503
6 · The paper itself

Abstract

The composition of the gut microbiome is altered in obesity and type 2 diabetes; however, it is not known whether these alterations are mediated by dietary factors or related to declines in metabolic health. To address this, cecal contents were collected from age-matched, chow-fed male University of California, Davis Type 2 Diabetes Mellitus (UCD-T2DM) rats before the onset of diabetes (prediabetic PD; n = 15), 2 wk recently diabetic (RD; n = 10), 3 mo (D3M; n = 11), and 6 mo (D6M; n = 8) postonset of diabetes. Bacterial species and functional gene counts were assessed by shotgun metagenomic sequencing of bacterial DNA in cecal contents, while metabolites were identified by gas chromatography-quadrupole time-off-flight-mass spectrometry. Metagenomic analysis showed a shift from Firmicutes species in early stages of diabetes (PD + RD) toward an enrichment of Bacteroidetes species in later stages of diabetes (D3M + D6M). In total, 45 bacterial species discriminated early and late stages of diabetes with 25 of these belonging to either Bacteroides or Prevotella genera. Furthermore, 61 bacterial gene clusters discriminated early and later stages of diabetes with elevations of enzymes related to stress response (e.g., glutathione and glutaredoxin) and amino acid, carbohydrate, and bacterial cell wall metabolism. Twenty-five cecal metabolites discriminated early vs. late stages of diabetes, with the largest differences observed in abundances of dehydroabietic acid and phosphate. Alterations in the gut microbiota and cecal metabolome track diabetes progression in UCD-T2DM rats when controlling for diet, age, and housing environment. Results suggest that diabetes-specific host signals impact the ecology and end product metabolites of the gut microbiome when diet is held constant.

Indexed as

MetabolomeAnimalsBacillotaBacteroidesCecumDiabetes Mellitus, Type 2DietDisease Models, AnimalGastrointestinal MicrobiomeMaleMetabolomicsMetagenomicsPrediabetic StateRatsRats, Sprague-Dawleydiabetesmetabolomicsmetagenomicsmicrobiota

Identifiers

PMID30016149
PMCPMC6293161

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.