ArticleBMC research notes2018
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system.
Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Wisent Somatic Cells Resist Reprogramming by the PiggyBac Transposon System: A Case Study Highlighting Methodological and Conservation Hurdles.International journal of molecular sciences · 2025Article
- Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat.Frontiers in nutrition · 2025Article
- Bovine Pluripotent Stem Cells: Current Status and Prospects.International journal of molecular sciences · 2024Review
- Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing.Stem cell reports · 2023Article
- Induced pluripotent stem cells from domesticated ruminants and their potential for enhancing livestock production.Frontiers in veterinary science · 2023Review
- Influence of Cell Type in In Vitro Induced Reprogramming in Cattle.Life (Basel, Switzerland) · 2022Article
- Efficient induction and sustenance of pluripotent stem cells from bovine somatic cells.Biology open · 2021Article
- Establishment of Bovine-Induced Pluripotent Stem Cells.International journal of molecular sciences · 2021Article
- Direct conversion of porcine primary fibroblasts into hepatocyte-like cells.Scientific reports · 2021Article
- Establishment of bovine expanded potential stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- The use of induced pluripotent stem cells in domestic animals: a narrative review.BMC veterinary research · 2020Review
- Induced pluripotent stem cells from farm animals.Journal of animal science · 2020Review
- Derivation of induced pluripotent stem cells from ferret somatic cells.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
- Stem cells on regenerative and reproductive science in domestic animals.Veterinary research communications · 2019Review
- A six-inhibitor culture medium for improving naïve-type pluripotency of porcine pluripotent stem cells.Cell death discovery · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
objectivesThe use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated.
resultsProtocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.