Evidence map›Paper›PMID 30075197›Full record

ArticleGene2018

ZBTB38, a novel regulator of autophagy initiation targeted by RB1CC1/FIP200 in spinal cord injury.

Jie Chen, Li Yan, Haosen Wang, Zengmeng Zhang, Daolun Yu, Chaofeng Xing, Jie Li, Honglin Li, Jun Li, Yafei Cai

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Jie ChenCollege of Life Sciences, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; The Secondary Hospital of Wuhu, Wuhu 241000, China.
Li YanDepartment of Radiation Oncology, Linyi People Hospital, 276003, China.
Haosen WangTaizhou 4th Hospital, Taizhou 235300, China.
Zengmeng ZhangCollege of Life Sciences, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China.
Daolun YuCollege of Life Sciences, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China.
Chaofeng XingCollege of Life Sciences, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China.
Jie LiCollege of Life Sciences, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China.
Honglin LiDepartment of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.
Jun LiCollege of Life Sciences, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China. Electronic address: lijunplant@163.com.
Yafei CaiCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: ycai@njau.edu.cn.
Anhui Normal University · CNNanjing Agricultural University · CNAugusta University · USLinyi People's Hospital · CN

Funding

Essential role of the Ufm1 E3 ligase in intestinal homeostasisR01DK113409 · NIDDK · AUGUSTA UNIVERSITY · PI LI, HONGLIN · 2017 to 2020
$1.4M
NIDDK NIH HHS R01 DK113409
6 · The paper itself

Abstract

Apoptosis is an important contributing factor in spinal cord injury (SCI). ZBTB38 is involved in the transcriptional regulation of multiple signaling pathways, is differentially expressed at different SCI stages, and may provide a therapeutic strategy for the treatment of patients with SCI. In this study, we found that autophagy is blocked in ZBTB38 knockdown SH-SY5Y cells and that the expression levels of LC3B II/I decreased and P62 increased. We used transcriptome high-throughput sequencing to identify the target in ZBTB38 knockdown cells. From the transcriptome profile, RB1CC1 (i.e., FIP200), a key component of the initiation machinery of autophagy (FIP200-ATG13-ULK1-ATG101), was found to decrease 4.2-fold following ZBTB38 knockdown. When RB1CC1-overexpressed plasmids were transfected into ZBTB38 knockdown cells, they rescued the phenotype of ZBTB38 knockdown cells. Cell proliferation and viability were significantly enhanced by RB1CC1 overexpression, and LC3B and P62 expression returned to their original levels. We also injected ZBTB38-overexpressed lentivirus into the injured center of the spinal cord and detected significant upregulation of RB1CC1 in the spinal cord. ZBTB38 overexpression can promote autophagy and partly rescue the secondary damage of SCI. Therefore, our findings provide a new strategy for the treatment of SCI.

Indexed as

AnimalsAutophagyAutophagy-Related ProteinsCell LineFemaleGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansMaleMiceProtein-Tyrosine KinasesRepressor ProteinsSpinal Cord InjuriesAutophagy-Related ProteinsProtein-Tyrosine KinasesRB1CC1 protein, humanRepressor ProteinsZBTB38 protein, humanAutophagyRB1CC1RNA sequencingSpinal Cord InjuryZBTB38

Identifiers

PMID30075197
PMCPMC7379175
OpenAlexW2887173426

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.