Evidence map›Paper›PMID 30151615›Full record

ArticleDiabetologia2018

Human adipose tissue-derived stromal cells act as functional pericytes in mice and suppress high-glucose-induced proinflammatory activation of bovine retinal endothelial cells.

Ghazaleh Hajmousa, Ewa Przybyt, Frederick Pfister, Genaro A Paredes-Juarez, Kondaiah Moganti, Stephanie Busch, Jeroen Kuipers, Ingeborg Klaassen, Marja J A van Luyn, Guido Krenning and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetologia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

  1. Exploring the Role of GLP-1 Agents in Managing Diabetic Foot Ulcers: A Narrative and Systematic Review.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
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  10. Autophagosomes Defeat Ferroptosis by Decreasing Generation and Increasing Discharge of Free FeAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Ghazaleh HajmousaDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713, GZ, Groningen, the Netherlands.ORCID http://orcid.org/0000-0001-7428-3312
Ewa PrzybytDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713, GZ, Groningen, the Netherlands.
Frederick Pfister5th Medical Department, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Genaro A Paredes-JuarezDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713, GZ, Groningen, the Netherlands.
Kondaiah MogantiInstitute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Stephanie Busch5th Medical Department, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Jeroen KuipersDepartment of Cell Biology, Molecular Imaging and Electron Microscopy, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
Ingeborg KlaassenOcular Angiogenesis Group, Departments of Ophthalmology and Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
Marja J A van LuynDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713, GZ, Groningen, the Netherlands.
Guido KrenningDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713, GZ, Groningen, the Netherlands.
Hans-Peter Hammes5th Medical Department, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Martin C HarmsenDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1 (EA11), 9713, GZ, Groningen, the Netherlands. m.c.harmsen@umcg.nl.
University Medical Center Groningen · NLHeidelberg University · DEAmsterdam UMC Location University of Amsterdam · NL

Funding

Deutsche Forschungsgemeinschaft (DFG): international research training group GRK 880 Vascular Medicine, Mannheim
6 · The paper itself

Abstract

aims/hypothesisThe immunomodulatory capacity of adipose tissue-derived stromal cells (ASCs) is relevant for next-generation cell therapies that aim to reverse tissue dysfunction such as that caused by diabetes. Pericyte dropout from retinal capillaries underlies diabetic retinopathy and the subsequent aberrant angiogenesis.

methodsWe investigated the pericytic function of ASCs after intravitreal injection of ASCs in mice with retinopathy of prematurity as a model for clinical diabetic retinopathy. In addition, ASCs influence their environment by paracrine signalling. For this, we assessed the immunomodulatory capacity of conditioned medium from cultured ASCs (ASC-Cme) on high glucose (HG)-stimulated bovine retinal endothelial cells (BRECs).

resultsASCs augmented and stabilised retinal angiogenesis and co-localised with capillaries at a pericyte-specific position. This indicates that cultured ASCs exert juxtacrine signalling in retinal microvessels. ASC-Cme alleviated HG-induced oxidative stress and its subsequent upregulation of downstream targets in an NF-κB dependent fashion in cultured BRECs. Functionally, monocyte adhesion to the monolayers of activated BRECs was also decreased by treatment with ASC-Cme and correlated with a decline in expression of adhesion-related genes such as SELE, ICAM1 and VCAM1. CONCLUSIONS/

interpretationThe ability of ASC-Cme to immunomodulate HG-challenged BRECs is related to the length of time for which ASCs were preconditioned in HG medium. Conditioned medium from ASCs that had been chronically exposed to HG medium was able to normalise the HG-challenged BRECs to normal glucose levels. In contrast, conditioned medium from ASCs that had been exposed to HG medium for a shorter time did not have this effect. Our results show that the manner of HG preconditioning of ASCs dictates their immunoregulatory properties and thus the potential outcome of treatment of diabetic retinopathy.

Indexed as

Adipose TissueAnimalsCattleCell AdhesionCells, CulturedCell SurvivalDiabetic RetinopathyEndothelial CellsEnzyme-Linked Immunosorbent AssayE-SelectinFemaleGlucoseHumansIntercellular Adhesion Molecule-1MaleMiceE-SelectinGlucoseIntercellular Adhesion Molecule-1Vascular Cell Adhesion Molecule-1Adipose tissue-derived stromal cellsDiabetic retinopathyHigh glucoseOxidative stress

Identifiers

PMID30151615
PMCPMC6182662
OpenAlexW2889523419

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.