ReviewAmerican journal of physiology. Renal physiology2018
Void spot assay: recommendations on the use of a simple micturition assay for mice.
Review in American journal of physiology. Renal physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 79 citations in OpenAlex.
- The Role of TRPV4 in Repeated Variate Stress-Induced Increases in Voiding Frequency and Somatic Sensitivity in Male and Female Mice.Journal of molecular neuroscience : MN · 2026Article
- PIEZO1-mediated mechanosensation links aging to bladder dysfunction.bioRxiv : the preprint server for biology · 2026Article
- Spoti-find: a novel, open-source void spot assay image analysis tool.American journal of physiology. Renal physiology · 2026Article
- Article
- Repurposing Dexmedetomidine: Early Pharmacological Hypothermia Enhances Neuroprotection and Improves Locomotor and Bladder Functional Recovery after Spinal Cord Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Protocol for inducing cystitis via direct instillation of acrolein into the bladder of mice.STAR protocols · 2026Article
- Diosgenin Promotes Bacterial Clearance in Bladder Epithelial Cells by Regulating Rab27b Expression: A Study Based on Virtual Screening and Experimental Validation.Infection and drug resistance · 2026Article
- Enhanced CHI3L1 promotes macrophage activation in persistent inflammatory events of ulcerative interstitial cystitis.Frontiers in immunology · 2026Article
- Piezo2 in sensory neurons contributes to inflammation-induced bladder overactivity and is associated with the effects of electroacupuncture.Frontiers in neuroscience · 2026Article
- Peripheral serotonin contributes to testosterone and estradiol induced urinary voiding dysfunction in adult male mice.American journal of clinical and experimental urology · 2026Article
- Sex differences in peripheral and local immune responses following spinal cord injury.Frontiers in immunology · 2026Article
- Investigation of image-guided in vivo irradiation on voiding patterns and bladder contractility in female mice.Scientific reports · 2025Article
- Robust noninvasive detection of hyperglycemia in mouse models of metabolic dysregulation using the novel Urination Index biomarker.Lab animal · 2025Article
- The RAGE Inhibitor TTP488 (Azeliragon) Improves Diabetic Bladder Dysfunction in Leptin-Deficient Obese Mice.Antioxidants (Basel, Switzerland) · 2025Article
- Void spot assay visualization optimization for use of Void Whizzard in rats (Rattus norvegicus).Physiological reports · 2025Article
- ML-UrineQuant: A machine learning program for identifying and quantifying mouse urine on absorbent paper.Physiological reports · 2025Article
- The mouse Social Frailty Index (mSFI): a novel behavioral assessment for impaired social functioning in aging mice.GeroScience · 2025Article
- Polyploid superficial uroepithelial bladder barrier cells express features of cellular senescence across the lifespan and are insensitive to senolytics.Aging cell · 2025Article
- Neuromodulation Improves Stress Urinary Incontinence-Like Deficits in Female Rabbits.IEEE open journal of engineering in medicine and biology · 2025Article
- Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Investigators have for decades used mouse voiding patterns as end points for studying behavioral biology. It is only recently that mouse voiding patterns were adopted for study of lower urinary tract physiology. The spontaneous void spot assay (VSA), a popular micturition assessment tool, involves placing a mouse in an enclosure lined by filter paper and quantifying the resulting urine spot pattern. The VSA has advantages of being inexpensive and noninvasive, but some investigators challenge its ability to distinguish lower urinary tract function from behavioral voiding. A consensus group of investigators who regularly use the VSA was established by the National Institutes of Health in 2015 to address the strengths and weaknesses of the assay, determine whether it can be standardized across laboratories, and determine whether it can be used as a surrogate for evaluating urinary function. Here we leverage experience from the consensus group to review the history of the VSA and its uses, summarize experiments to optimize assay design for urinary physiology assessment, and make best practice recommendations for performing the assay and analyzing its results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.