Evidence map›Paper›PMID 30257879›Full record

ArticleMolecular & cellular proteomics : MCP2019

Identification of Novel Natural Substrates of Fibroblast Activation Protein-alpha by Differential Degradomics and Proteomics.

Hui Emma Zhang, Elizabeth J Hamson, Maria Magdalena Koczorowska, Stefan Tholen, Sumaiya Chowdhury, Charles G Bailey, Angelina J Lay, Stephen M Twigg, Quintin Lee, Ben Roediger and 6 more

Registry-linked trialOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07215182 ([68Ga]Ga-FAPI PET/CT for Response Evaluation During Immune Checkpoint Inhibitor Therapy in Malignant Melanoma), which is not on this map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07215182 phase2recruitingnot on this mapstarted 2025, after this paper: background citation

[68Ga]Ga-FAPI PET/CT for Response Evaluation During Immune Checkpoint Inhibitor Therapy in Malignant Melanoma

TypeinterventionalSponsorBarbara Malene FischerRan2025 to 2027Enrolled20ConditionsMalignant Melanoma Stage IVArms[68Ga]Ga-FAPI-46
3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 64 citations in OpenAlex.

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  10. Exploring CTRP6: a biomarker and therapeutic target in metabolic diseases.American journal of physiology. Endocrinology and metabolism · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 2 countries.

Hui Emma ZhangFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Elizabeth J HamsonFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Maria Magdalena Koczorowska¶Institute for Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany.
Stefan Tholen¶Institute for Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany.
Sumaiya ChowdhuryFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Charles G BaileyFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Angelina J LayFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Stephen M Twigg§Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia;; ‖Charles Perkins Centre, the University of Sydney, New South Wales, 2006, Australia.
Quintin LeeFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Ben RoedigerFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Martin L Biniossek¶Institute for Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany.
Matthew B O'Rourke‖Charles Perkins Centre, the University of Sydney, New South Wales, 2006, Australia;; **Proteomics Core Facility, University of Technology Sydney, New South Wales, 2007, Australia.
Geoffrey W McCaughanFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Fiona M KeaneFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia.
Oliver Schilling‡‡Institute of Surgical Pathology, University Medical Center - University of Freiburg, Freiburg, Germany;; §§BIOSS Centre for Biological Signaling Studies, University of Freiburg, Freiburg, Germany;; ¶¶German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address: oliver.schilling@mol-med.uni-freiburg.de.
Mark D GorrellFrom the ‡Centenary Institute, the University of Sydney, Locked Bag No.6, Newtown, New South Wales, 2042, Australia;; §Sydney Medical School, the University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia;; ‖Charles Perkins Centre, the University of Sydney, New South Wales, 2006, Australia;. Electronic address: m.gorrell@centenary.usyd.edu.au.
The University of Sydney · AUUniversity of Freiburg · DEUniversity of Technology Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fibroblast activation protein-alpha (FAP) is a cell-surface transmembrane-anchored dimeric protease. This unique, constitutively active serine protease has both dipeptidyl aminopeptidase and endopeptidase activities and can hydrolyze the post-proline bond. FAP expression is very low in adult organs but is upregulated by activated fibroblasts in sites of tissue remodeling, including fibrosis, atherosclerosis, arthritis and tumors. To identify the endogenous substrates of FAP, we immortalized primary mouse embryonic fibroblasts (MEFs) from FAP gene knockout embryos and then stably transduced them to express either enzymatically active or inactive FAP. The MEF secretomes were then analyzed using degradomic and proteomic techniques. Terminal amine isotopic labeling of substrates (TAILS)-based degradomics identified cleavage sites in collagens, many other extracellular matrix (ECM) and associated proteins, and lysyl oxidase-like-1, CXCL-5, CSF-1, and C1qT6, that were confirmed

Indexed as

AdipokinesAmino Acid OxidoreductasesAnimalsCell Culture TechniquesCell LineChemokine CXCL5EndopeptidasesFibroblast Activation Protein AlphaFibroblastsGelatinasesGene Knockout TechniquesHumansMacrophage Colony-Stimulating FactorMembrane ProteinsMiceProtein Interaction MapsAdipokinesAmino Acid OxidoreductasesChemokine CXCL5CSF1 protein, mouseCTRP6 protein, mouseCxcl5 protein, mouseEndopeptidasesFibroblast Activation Protein AlphaGelatinasesLoxl1 protein, mouseMacrophage Colony-Stimulating FactorMembrane ProteinsSerine EndopeptidasesCoagulationCytokinesDegradomicsEndopeptidasesExtracellular MatrixFibroblast Activation ProteinFibroblastsProteasesSILACSubstrate Identification

Identifiers

PMID30257879
PMCPMC6317473
OpenAlexW2894425317

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.