Evidence map›Paper›PMID 30264516›Full record

ArticleJournal of periodontal research2019

Activity of two hyaluronan preparations on primary human oral fibroblasts.

Maria B Asparuhova, Deniz Kiryak, Meizi Eliezer, Deyan Mihov, Anton Sculean

4 registry-linked trialsOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of periodontal research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 4 registered trials, which are not on this map. Cited by 71 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed, 5 pooled it
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04865952 nacompletednot on this mapstarted 2021, after this paper: background citation

Effect of Hyaluronic Acid Application in Gene Expression and Cellular Behavior 24-hours After Injury

TypeinterventionalSponsorUniversity of Roma La SapienzaRan2021 to 2021Enrolled8ConditionsWound Heal, Wound Surgical, Oral Soft Tissue Conditions, Hyaluronic AcidArmsperiodontal surgery + 24 hr buccal attached gingiva (G) biopsy, HA application
NCT06914518 nanot yet recruitingnot on this mapstarted 2025, after this paper: background citation

Polynucleotide and Hyaluronic Acid-based Gel as Adjunctive Therapy to Non- Surgical Treatment of Intraosseous Peri-implant Defect. A 6-month Randomized Clinical Trial

TypeinterventionalSponsorUniversitat Internacional de CatalunyaRan2025 to 2026Enrolled32ConditionsPeri-implantitisArmsviscoelastic gel containing a fixed combination of natural origin PN (10 mg/ml, 1%) and HA with molecular weight > 1500 kDa (10 mg/ml, 1%), Non-Surgical Intervention
NCT07458685 nacompletednot on this mapstarted 2022, after this paper: background citation

Efficacy of Cross-linked Hyaluronic Acid in Improving Healing After Free Gingival Graft: a Randomized Controlled Clinical Trial

TypeinterventionalSponsorUniversity of MilanRan2022 to 2025Enrolled20ConditionsPalatal Donor Site Wound Healing, Palatal WoundArmsTest - xHyA, Negative control
NCT07529340 nanot yet recruitingnot on this mapstarted 2026, after this paper: background citation

Effectiveness of Hyaluronic Acid Application in Coronally Advanced Flap Combined With a Subepithelial Connective Tissue Graft Versus Coronally Advanced Flap Combined With a Subepithelial Connective Tissue Graft Alone: A Clinical Trial

TypeinterventionalSponsorUniversidade do PortoRan2026 to 2027Enrolled34ConditionsGingival Recession, Mucogingival SurgeryArmsCoronally Advanced Flap with Connective Tissue Graft, Cross-linked Hyaluronic Acid Gel
3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 5 syntheses or guidelines pooled it, 89 citations in OpenAlex.

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11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Maria B AsparuhovaLaboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland.ORCID http://orcid.org/0000-0003-2773-1324
Deniz KiryakLaboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Meizi EliezerDepartment of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Deyan MihovBiozentrum, University of Basel, Basel, Switzerland.
Anton SculeanDepartment of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
University of Bern · CHUniversity of Basel · CH

Funding

Regedent, Zürich, Switzerland
6 · The paper itself

Abstract

BACKGROUND AND

objectiveThe potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration. MATERIAL AND

methodsMetabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing-related gene expression and activation of signaling kinases were analyzed by qRT-PCR and immunoblotting, respectively.

resultsThe investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migratory abilities. They enhanced expression of genes encoding type III collagen and transforming growth factor-β3, characteristic of scarless wound healing. The HAs upregulated the expression of genes encoding pro-proliferative, pro-migratory, and pro-inflammatory factors, with only a moderate effect on the latter in gingival fibroblasts. In palatal but not gingival fibroblasts, an indirect effect of HA on the expression of matrix metalloproteinases 2 and 3 was detected, potentially exerted through induction of pro-inflammatory cytokines. Finally, our data pointed on Akt, Erk1/2 and p38 as the signaling molecules whereby the HAs exert their effects on oral fibroblasts.

conclusionBoth investigated HA formulations are biocompatible and enhance the proliferative, migratory and wound healing properties of cell types involved in soft tissue wound healing following regenerative periodontal surgery. Our data further suggest that in gingival tissues, the HAs are not likely to impair the healing process by prolonging inflammation or causing excessive MMP expression at the repair site.

Indexed as

Cell MovementCell ProliferationCells, CulturedCollagen Type IIIConnective TissueDrug CompoundingFibroblastsGene ExpressionGingivaHumansHyaluronic AcidMatrix Metalloproteinase 2Matrix Metalloproteinase 3PalateRegenerationRegenerative EndodonticsCollagen Type IIIHyaluronic AcidMatrix Metalloproteinase 2Matrix Metalloproteinase 3Transforming Growth Factor beta3gene expressiongrowth factorshyaluronic acidoral soft tissue wound healingpro-inflammatory cytokines

Identifiers

PMID30264516
PMCPMC6586051
OpenAlexW2893517936

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.