ArticleScientific reports2018
A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
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- A general strategy for high-efficiency live bacteria imaging and targeted phototherapy.Chemical science · 2026Article
- Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.Angewandte Chemie (International ed. in English) · 2026Article
- Article
- A constricted mitochondrial morphology formed during respiration.Nature communications · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- A Photoactivatable Plasma Membrane Probe Based on a Self-Triggered Photooxidation Cascade for Live Cell Super-Resolution Microscopy.Angewandte Chemie (International ed. in English) · 2025Article
- Emerging Approaches for Studying Lipid Dynamics, Metabolism, and Interactions in Cells.Annual review of biochemistry · 2025Review
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance.Chemical & biomedical imaging · 2024Review
- Anisotropic DBSCAN for 3D SMLM Data Clustering.The journal of physical chemistry. B · 2024Article
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Contribution of different macromolecules to the diffusion of a 40 nm particle in Escherichia coli.Biophysical journal · 2024Article
- Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.Nature communications · 2024Article
- The extracellular vesicle proteomes of Sorghum bicolor and Arabidopsis thaliana are partially conserved.Plant physiology · 2024Article
- Expanding the microbiologist toolboxMicrobiology spectrum · 2024Article
- ULK1 forms distinct oligomeric states and nanoscopic structures during autophagy initiation.Science advances · 2023Article
- Three-dimensional structured illumination microscopy with enhanced axial resolution.Nature biotechnology · 2023Article
- Unprecedented perspectives on the application of CinNapht fluorophores provided by a "late-stage" functionalization strategy.Chemical science · 2023Article
- Interactions of Nanomaterials with Gut Microbiota and Their Applications in Cancer Therapy.Sensors (Basel, Switzerland) · 2023Review
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Maintenance of the bacterial homeostasis initially emanates from interactions between proteins and the bacterial nucleoid. Investigating their spatial correlation requires high spatial resolution, especially in tiny, highly confined and crowded bacterial cells. Here, we present super-resolution microscopy using a palette of fluorescent labels that bind transiently to either the membrane or the nucleoid of fixed E. coli cells. The presented labels are easily applicable, versatile and allow long-term single-molecule super-resolution imaging independent of photobleaching. The different spectral properties allow for multiplexed imaging in combination with other localisation-based super-resolution imaging techniques. As examples for applications, we demonstrate correlated super-resolution imaging of the bacterial nucleoid with the position of genetic loci, of nascent DNA in correlation to the entire nucleoid, and of the nucleoid of metabolically arrested cells. We furthermore show that DNA- and membrane-targeting labels can be combined with photoactivatable fluorescent proteins and visualise the nano-scale distribution of RNA polymerase relative to the nucleoid in drug-treated E. coli cells.
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