ArticlePlant signaling & behavior2018
Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato.
Article in Plant signaling & behavior, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
90 citing papers in PubMed, 1 synthesis or guideline pooled it, 231 citations in OpenAlex.
- Genome editing for vegetable crop improvement: Challenges and future prospects.Frontiers in genetics · 2022Pooled it
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- A Modified Cas9 Scaffold Allows Extension of the Virus-Induced Gene Editing Technology to the Large Potyvirus Genus.Plant biotechnology journal · 2026Article
- Enhanced Rice Yellow Mottle Virus Resistance via CRISPR/Cas9-Targeted Mutagenesis of the Rice eIF(iso)4G Gene.Molecular plant pathology · 2026Article
- Evaluation of CRISPR/Cas9 based single and multiplex gRNAs against ChiLCV in tobacco.Journal, genetic engineering & biotechnology · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Genome editing‑based strategies to combat geminiviruses: CRISPR/Cas9 and emerging high‑fidelity tools.Archives of microbiology · 2026Review
- From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026Review
- Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.BMC plant biology · 2026Article
- CRISPR-Cas systems for plant virus management: detection, surveillance, and host resistance.Frontiers in plant science · 2026Review
- Unveiling the landscape of plant virology in Saudi Arabia: seven decades of progress and future directions toward Vision 2030.Frontiers in plant science · 2026Review
- Genome editing and regeneration pipeline for engineering disease resistance in tomato using CRISPR/Cas9.Frontiers in plant science · 2026Article
- Detection method for CRISPR-Cas9 edited tomato with single nucleotide deletion using multiplex probe-based real-time PCR and confirmation of non-transgenicity: A case study.Journal, genetic engineering & biotechnology · 2025Article
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments.Functional & integrative genomics · 2025Review
- Breeding vegetables for whitefly resistance: past, present, and future in the AI era.Frontiers in plant science · 2025Review
- Marker assisted pyramiding of major resistance genes of tomato leaf curl and late blight diseases for stabilising tomato production.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants.Plants (Basel, Switzerland) · 2024Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Geminiviruses and Food Security: A Molecular Genetics Perspective for Sustainable Agriculture in Africa.Plants (Basel, Switzerland) · 2024Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.