Evidence map›Paper›PMID 30315899›Full record

ArticleGenomics2019

Identification of DNA methylation regulated novel host genes relevant to inhibition of virus replication in porcine PK15 cell using double stranded RNA mimics and DNA methyltransferase inhibitor.

Xiaoshuo Wang, Hong Ao, Minyan Song, Lijing Bai, Weiyong He, Chuduan Wang, Ying Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Xiaoshuo WangNational Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China; Department of Animal Husbandry and Veterinary Technology, Vocational Technical College, Inner Mongolia Argriculture University, Baotou 014109, China.
Hong AoState Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Chinese Academy of Agricultural Sciences, Institute of Animal Science, Beijing 100193, China.
Minyan SongNational Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China.
Lijing BaiState Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Chinese Academy of Agricultural Sciences, Institute of Animal Science, Beijing 100193, China.
Weiyong HeState Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing 100193, China.
Chuduan WangNational Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: cdwang@cau.edu.cn.
Ying YuNational Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: yuying@cau.edu.cn.
China Agricultural University · CNChinese Academy of Agricultural Sciences · CNBaotou Teachers College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During RNA viruses's replication, double-stranded RNA (dsRNA) is normally produced and induce host innate immune response. Most of gene activation due cytokine mediated but which are due to methylation mediated is still unknown. In the study, DNA methylome was integrated with our previous transcriptome data to investigate the differentially methylated regions and genes using MeDIP-chip technology. We found that the transcriptional expressions of 15, 37 and 18 genes were negatively related with their promoter DNA methylation levels in the cells treated by PolyI:C, Aza-CdR, as well as PolyI:C plus Aza-CdR, respectively, compared with the untreated cells. GO analysis revealed hypo-methylated genes (BNIP3L and CDK9) and a hyper-methylated gene (ZC3HAV1) involved in the host response to viral replication. Our results suggest that these novel genes targeted by DNA methylation can be potential markers relevant to virus replication and host innate immune response to set up a medical model of infectious diseases.

Indexed as

DNA MethylationAnimalsAzacitidineCells, CulturedDNA Modification MethylasesEnzyme InhibitorsGene Expression RegulationGenomeKidneyPromoter Regions, GeneticRNA, Double-StrandedSwineTranscriptomeVirus ReplicationAzacitidineDNA Modification MethylasesEnzyme InhibitorsRNA, Double-StrandedAza-CdRDNA methylomeMethylation targeted genesPolyI:CTranscriptomeViral replication

Identifiers

PMID30315899
PMCPMC7125705
OpenAlexW2896972911

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.