Evidence map›Paper›PMID 30317500›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Single-Molecule Optical Tweezers Study of Regulated SNARE Assembly.

Lu Ma, Junyi Jiao, Yongli Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 2 countries.

Lu MaBeijing National Laboratory for Condensed Matter Physics and CAS Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China.
Junyi JiaoDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA.
Yongli ZhangDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT, USA. yongli.zhang@yale.edu.
Chinese Academy of Sciences · CNUniversity of New Haven · USYale University · US

Funding

Single-molecule manipulation of SNAREsR01GM093341 · NIGMS · YALE UNIVERSITY · PI ZHANG, YONGLI · 2010 to 2018
$3.1M
Common role of Sec1p/Munc18 proteins in SNARE assembly and membrane fusionR01GM120193 · NIGMS · YALE UNIVERSITY · PI ZHANG, YONGLI · 2017 to 2018
$709k
NIGMS NIH HHS R01 GM093341NIGMS NIH HHS R01 GM120193
6 · The paper itself

Abstract

Intracellular membrane fusion mediates material and information exchange among different cells or cellular compartments with high accuracy and spatiotemporal resolution. Fusion is driven by ordered folding and assembly of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptors (SNAREs) and regulated by many other proteins. Understanding regulated SNARE assembly is key to dissecting mechanisms and physiologies of various fusion processes and their associated diseases. Yet, it remains challenging to study regulated SNARE assembly using traditional ensemble-based experimental approaches. Here, we describe our new method to measure the energy and kinetics of neuronal SNARE assembly in the presence of α-SNAP, using a single-molecule manipulation approach based on high-resolution optical tweezers. Detailed experimental protocols and methods of data analysis are shown. This approach can be widely applied to elucidate the effects of regulatory proteins on SNARE assembly and membrane fusion.

Indexed as

Optical TweezersBiotinylationCross-Linking ReagentsKineticsMembrane FusionMicrofluidic Analytical TechniquesProtein BindingProtein FoldingProtein Structure, QuaternaryRecombinant ProteinsSingle Molecule ImagingSNARE ProteinsSoluble N-Ethylmaleimide-Sensitive Factor Attachment ProteinsCross-Linking ReagentsRecombinant ProteinsSNARE ProteinsSoluble N-Ethylmaleimide-Sensitive Factor Attachment ProteinsEnergy landscapeHidden Markov modelingNSFOptical tweezersSingle-molecule manipulationSNAPSNARE assembly and disassembly

Identifiers

PMID30317500
PMCPMC6441361
OpenAlexW2897020895

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.