Evidence mapPaperPMID 30364784Full record

ArticleCellular and molecular gastroenterology and hepatology2018

Dynamic Formation of Microvillus Inclusions During Enterocyte Differentiation in

Mohammed H Mosa, Ophélie Nicolle, Sophia Maschalidi, Fernando E Sepulveda, Aurelien Bidaud-Meynard, Constantin Menche, Birgitta E Michels, Grégoire Michaux, Geneviève de Saint Basile, Henner F Farin

Open access · goldAbstract read
In one paragraph

Article in Cellular and molecular gastroenterology and hepatology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Downregulation of V-ATPase VCellular and molecular gastroenterology and hepatology · 2024
    Article
  5. Article
  6. Review
  7. Article
  8. The Role of Metal Oxide Nanoparticles,Environmental science. Nano · 2020
    Article
  9. Article
  10. Human Microphysiological Models of Intestinal Tissue and Gut Microbiome.Frontiers in bioengineering and biotechnology · 2020
    Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Modeling Microvillus Inclusion Formation In Vitro.Cellular and molecular gastroenterology and hepatology · 2018
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Mohammed H MosaGerman Cancer Consortium (Deutsches Konsortium für Translationale Krebsforschung), Heidelberg, Germany.
Ophélie NicolleUniversity Rennes, Centre national de la recherche scientifique, Institut de Génétique et Développement de Rennes UMR6290, Rennes, France.
Sophia MaschalidiINSERM UMR1163, Laboratory of Normal and Pathological Homeostasis of the Immune System, Paris, France.
Fernando E SepulvedaINSERM UMR1163, Laboratory of Normal and Pathological Homeostasis of the Immune System, Paris, France.
Aurelien Bidaud-MeynardUniversity Rennes, Centre national de la recherche scientifique, Institut de Génétique et Développement de Rennes UMR6290, Rennes, France.
Constantin MencheGeorg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main, Germany.
Birgitta E MichelsGerman Cancer Consortium (Deutsches Konsortium für Translationale Krebsforschung), Heidelberg, Germany.
Grégoire MichauxUniversity Rennes, Centre national de la recherche scientifique, Institut de Génétique et Développement de Rennes UMR6290, Rennes, France.
Geneviève de Saint BasileINSERM UMR1163, Laboratory of Normal and Pathological Homeostasis of the Immune System, Paris, France.
Henner F FarinGerman Cancer Consortium (Deutsches Konsortium für Translationale Krebsforschung), Heidelberg, Germany.
Deutschen Konsortium für Translationale Krebsforschung · DEInstitut de génétique et de développement de Rennes · FRInstitut des Maladies Génétiques Imagine · FRCentre de Référence Déficits Immunitaires Héréditaires · FRGeorg Speyer Haus · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background & Aims: Microvillus inclusion disease (MVID) is a congenital intestinal malabsorption disorder caused by defective apical vesicular transport. Existing cellular models do not fully recapitulate this heterogeneous pathology. The aim of this study was to characterize 3-dimensional intestinal organoids that continuously generate polarized absorptive cells as an accessible and relevant model to investigate MVID. Methods: Intestinal organoids from Results: Loss of Conclusions: We show that prolonged enterocyte-specific differentiation is required to recapitulate the entire spectrum of MVID. Primary organoids can provide a powerful model for this heterogeneous pathology. Formation of microvillus inclusions from multiple membrane sources showed an unexpected dynamic of the enterocyte brush border.

Indexed as

Cell DifferentiationActinsAnimalsCell NucleusEnterocytesHumansIntestinesLysosomesMalabsorption SyndromesMice, KnockoutMicrovilliMucolipidosesMunc18 ProteinsOrganoidsActinsMunc18 ProteinsSTXBP2 protein, humanStxbp2 protein, mouse3D, 3-dimensionalApical Vesicular TransportBrush Border FormationDAPI, 4′,6-diamidino-2-phenylindoleDisease ModelingEGFP, enhanced green fluorescent proteinFHL5, familial hemophagocytic lymphohistiocytosis type 5IWP-2, inhibitor of WNT production-2KO, knock-outMicrovillus AtrophyMVID, microvillus inclusion diseaseMVIs, microvillus inclusionsPBS, phosphate-buffered salineStx3, syntaxin 3STXBP2, syntaxin binding protein 2TEM, transmission electron microscopyVPA, valproic acidWT, wild-type

Identifiers

PMID30364784
PMCPMC6198061
OpenAlexW2887711017

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.