Evidence map›Paper›PMID 30415271›Full record

ArticleALTEX2019

Characterizing sources of variability in zebrafish embryo screening protocols.

Jon T Hamm, Patricia Ceger, David Allen, Matt Stout, Elizabeth A Maull, Greg Baker, Amy Zmarowski, Stephanie Padilla, Edward Perkins, Antonio Planchart and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ALTEX, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
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  17. Exploring the Influence of Experimental Design on Toxicity Outcomes in Zebrafish Embryo Tests.Toxicological sciences : an official journal of the Society of Toxicology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jon T HammIntegrated Laboratory Systems, Research Triangle Park, NC, USA.
Patricia CegerIntegrated Laboratory Systems, Research Triangle Park, NC, USA.
David AllenIntegrated Laboratory Systems, Research Triangle Park, NC, USA.
Matt StoutDivision of the National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
Elizabeth A MaullNational Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
Greg BakerBattelle, Life Sciences Research, Columbus, OH, USA.
Amy ZmarowskiBattelle, Life Sciences Research, Columbus, OH, USA.
Stephanie PadillaIntegrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA.
Edward PerkinsUnited States Army Engineer Research and Development Center, Vicksburg, MS, USA.
Antonio PlanchartDepartment of Biological Sciences and Center for Human Health and the Environment, North Carolina State University, Raleigh, NC, USA.
Donald StedmanPfizer Pharmaceuticals, New London/Norwich, CT, USA.
Tamara TalIntegrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA.
Robert L TanguayDepartment of Environmental & Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
David C VolzDepartment of Environmental Sciences, University of California, Riverside, CA, USA.
Mitch S WilbanksUnited States Army Engineer Research and Development Center, Vicksburg, MS, USA.
Nigel J WalkerDivision of the National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.

Funding

Toxicological Assessments for the National Toxicology ProgramZIAES103316 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI WALKER, NIGEL J · 2016 to 2019
$31.9M
NIEHS NIH HHS HHSN273201500010C
6 · The paper itself

Abstract

There is a need for fast, efficient, and cost-effective hazard identification and characterization of chemical hazards. This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. A Collaborative Workshop on Aquatic Models and 21st Century Toxicology identified the lack of appropriate and consistent testing protocols as a challenge to the broader application of the zebrafish embryo model. The National Toxicology Program established the Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT) initiative to address the lack of consistent testing guidelines and identify sources of variability for zebrafish-based assays. This report summarizes initial SEAZIT information-gathering efforts. Investigators in academic, government, and industry laboratories that routinely use zebrafish embryos for chemical toxicity testing were asked about their husbandry practices and standard protocols. Information was collected about protocol components including zebrafish strains, feed, system water, disease surveillance, embryo exposure conditions, and endpoints. Literature was reviewed to assess issues raised by the investigators. Interviews revealed substantial variability across design parameters, data collected, and analysis procedures. The presence of the chorion and renewal of exposure media (static versus static-renewal) were identified as design parameters that could potentially influence study outcomes and should be investigated further with studies to determine chemical uptake from treatment solution into embryos. The information gathered in this effort provides a basis for future SEAZIT activities to promote more consistent practices among researchers using zebrafish embryos for toxicity evaluation.

Indexed as

Embryo, NonmammalianAnimalsChorionDrug Evaluation, PreclinicalEmbryonic DevelopmentHigh-Throughput Screening AssaysToxicity TestsZebrafishdevelopmental toxicityhigh-throughput screeningNon-mammalian species

Identifiers

PMID30415271
PMCPMC10424490

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.