ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2019
Enhancing grain size in durum wheat using RNAi to knockdown GW2 genes.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Identification of stable QTL for yield-related traits in a durum wheat Svevo × Zavitan RIL population.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Genome-Wide Identification of Ovarian Tumor Proteases Gene Family and Knockout ofPlants (Basel, Switzerland) · 2026Article
- Nuclear factor-Y-polycomb repressive complex2 dynamically orchestrates starch and seed storage protein biosynthesis in wheat.The Plant cell · 2024Article
- Molecular mechanisms controlling grain size and weight and their biotechnological breeding applications in maize and other cereal crops.Journal of advanced research · 2024Review
- Integrated Transcriptomic and Proteomic Characterization of a Chromosome Segment Substitution Line Reveals the Regulatory Mechanism Controlling the Seed Weight in Soybean.Plants (Basel, Switzerland) · 2024Article
- Image facilitated assessment of intra-spike variation in grain size in wheat under high temperature and drought stress.Scientific reports · 2023Article
- Genomics for Yield and Yield Components in Durum Wheat.Plants (Basel, Switzerland) · 2023Review
- Identification of Potential QTLs Related to Grain Size in Rice.Plants (Basel, Switzerland) · 2023Article
- The Identification of a Yield-Related Gene Controlling Multiple Traits Using GWAS in Sorghum (Plants (Basel, Switzerland) · 2023Article
- Interaction between Sulfate and Selenate in Tetraploid Wheat (International journal of molecular sciences · 2023Article
- Functional analysis of polyphenol oxidase 1 gene in common wheat.Frontiers in plant science · 2023Article
- Natural allelic variation of GmST05 controlling seed size and quality in soybean.Plant biotechnology journal · 2022Article
- Genes Impacting Grain Weight and Number in Wheat (Plants (Basel, Switzerland) · 2022Review
- Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand.International journal of molecular sciences · 2022Review
- Genome-Wide Analysis of theFrontiers in plant science · 2022Article
- Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- The decreased expression of GW2 homologous genes contributed to the increased grain width and thousand‑grain weight in wheat-Dasypyrum villosum 6VS·6DL translocation lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- A 24,482-bp deletion is associated with increased seed weight in Brassica napus L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Biotechnological Resources to Increase Disease-Resistance by Improving Plant Immunity: A Sustainable Approach to Save Cereal Crop Production.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageKnocking down GW2 enhances grain size by regulating genes encoding the synthesis of cytokinin, gibberellin, starch and cell wall. Raising crop yield is a priority task in the light of the continuing growth of the world's population and the inexorable loss of arable land to urbanization. Here, the RNAi approach was taken to reduce the abundance of Grain Weight 2 (GW2) transcript in the durum wheat cultivar Svevo. The effect of the knockdown was to increase the grains' starch content by 10-40%, their width by 4-13% and their surface area by 3-5%. Transcriptomic profiling, based on a quantitative real-time PCR platform, revealed that the transcript abundance of genes encoding both cytokinin dehydrogenase 1 and the large subunit of ADP-glucose pyrophosphorylase was markedly increased in the transgenic lines, whereas that of the genes encoding cytokinin dehydrogenase 2 and gibberellin 3-oxidase was reduced. A proteomic analysis of the non-storage fraction extracted from mature grains detected that eleven proteins were differentially represented in the transgenic compared to wild-type grain: some of these were involved, or at least potentially involved, in cell wall development, suggesting a role of GW2 in the regulation of cell division in the wheat grain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.