ArticleMolecular medicine reports2019
Hypoxia‑induced miR‑210 contributes to apoptosis of mouse spermatocyte GC‑2 cells by targeting Kruppel‑like factor 7.
Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Paraxanthine and azilsartan attenuate gentamicin-induced renal fibrosis via modulation of TGF-β1/Smad3/7 signaling and miRNA-21/miRNA-200b expression.Journal of translational medicine · 2026Article
- The microRNA-mediated apoptotic signaling axis in male reproduction: a possible and targetable culprit in male infertility.Cell biology and toxicology · 2025Review
- SIRT3 inhibition suppresses hypoxia-inducible factor 1α signaling and alleviates hypoxia-induced apoptosis of type B spermatogonia GC-2 cells.FEBS open bio · 2023Article
- Unveiling caspase-2 regulation by non-coding RNAs.Cell death & disease · 2022Review
- miRNA Pattern in Hypoxic Microenvironment of Kidney Cancer-Role of PTEN.Biomolecules · 2022Article
- Krüppel-like factor 7 attenuates hippocampal neuronal injury after traumatic brain injury.Neural regeneration research · 2022Article
- Comprehensive Analysis and Reinforcement Learning of Hypoxic Genes Based on Four Machine Learning Algorithms for Estimating the Immune Landscape, Clinical Outcomes, and Therapeutic Implications in Patients With Lung Adenocarcinoma.Frontiers in immunology · 2022Article
- Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress.Frontiers in molecular biosciences · 2022Article
- miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner.Biomedicines · 2021Article
- Effects of Environmental and Pathological Hypoxia on Male Fertility.Frontiers in cell and developmental biology · 2021Review
- Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis.Stem cell research & therapy · 2020Article
- Article
- Seminal plasma miR-210-3p induces spermatogenic cell apoptosis by activating caspase-3 in patients with varicocele.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to investigate the underlying mechanisms of hypoxia‑induced microRNA (miR)‑210 effects on mouse GC‑2spd (GC‑2) cells. GC‑2 cells were subjected to hypoxia or normoxia for 12, 24, 48 and 72 h. Apoptosis of GC‑2 cells was detected using terminal deoxynucleotidyl‑transferase‑meditated dUTP nick end labeling and flow cytometry. Reverse transcription‑quantitative polymerase chain reaction was performed to analyze the expression of miR‑210. Hypoxia‑inducible factor‑1α (HIF‑1α), caspase‑3, B‑cell lymphoma 2, apoptosis regulator BAX and Kruppel‑like factor 7 (KLF7) protein expression levels were detected by western blotting. Luciferase reporter gene assays were used to assess the targeting effects of miR‑210 on KLF7. Hypoxia induced GC‑2 cell apoptosis and increased the expression of HIF‑1α and pro‑apoptotic proteins; however, decreased anti‑apoptotic protein expression levels. Furthermore, hypoxia resulted in the upregulation of miR‑210 in GC‑2 cells. HIF‑1α and miR‑210 were involved in the apoptosis of GC‑2 cells by mediating the expression of apoptosis‑associated proteins. Furthermore, KLF7 was directly targeted by miR‑210 to influence the apoptosis of GC‑2 cells subjected to hypoxia. The results suggested that hypoxia‑induced miR‑210 stimulated the activation of the apoptosis signaling pathway and contributed to the apoptosis of GC‑2 cells by targeting KLF7.
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