ArticlemSphere2018
Label-Free Digital Holo-tomographic Microscopy Reveals Virus-Induced Cytopathic Effects in Live Cells.
Article in mSphere, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Label-free quantification of early virus-induced cellular responses by digital holographic tomography.mSphere · 2026Article
- A Benchmark for Virus Infection Reporter Virtual Staining in Fluorescence and Brightfield Microscopy.Scientific data · 2025Article
- Characterization of a Single-Capture Bright-Field and Off-Axis Digital Holographic Microscope for Biological Applications.Sensors (Basel, Switzerland) · 2025Article
- AI-based classification of anticancer drugs reveals nucleolar condensation as a predictor of immunogenicity.Molecular cancer · 2024Article
- Article
- Label-Free Three-Dimensional Morphological Characterization of Cell Death Using Holographic Tomography.Sensors (Basel, Switzerland) · 2024Article
- Article
- Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research.Discover nano · 2024Review
- A weak-labelling and deep learning approach for in-focus object segmentation in 3D widefield microscopy.Scientific reports · 2023Article
- Label-free microscopy for virus infections.Microscopy (Oxford, England) · 2023Article
- Therapeutic Potential of Medicinal Plants against Dengue Infection: A Mechanistic Viewpoint.ACS omega · 2022Review
- Detection of Cytopathic Effects Induced by Influenza, Parainfluenza, and Enterovirus Using Deep Convolution Neural Network.Biomedicines · 2021Article
- Virus Infection Variability by Single-Cell Profiling.Viruses · 2021Review
- Metabolic Signatures ofBiology · 2021Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytopathic effects (CPEs) are a hallmark of infections. CPEs are difficult to observe due to phototoxicity from classical light microscopy. We report distinct patterns of virus infections in live cells using digital holo-tomographic microscopy (DHTM). DHTM is label-free and records the phase shift of low-energy light passing through the specimen on a transparent surface with minimal perturbation. DHTM measures the refractive index (RI) and computes the refractive index gradient (RIG), unveiling optical heterogeneity in cells. We find that vaccinia virus (VACV), herpes simplex virus (HSV), and rhinovirus (RV) infections progressively and distinctly increased RIG. VACV infection, but not HSV and RV infections, induced oscillations of cell volume, while all three viruses altered cytoplasmic membrane dynamics and induced apoptotic features akin to those caused by the chemical compound staurosporine. In sum, we introduce DHTM for quantitative label-free microscopy in infection research and uncover virus type-specific changes and CPE in living cells with minimal interference.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.