Evidence mapPaperPMID 30557366Full record

ArticlePloS one2018

GSK-3 inhibitors enhance TRAIL-mediated apoptosis in human gastric adenocarcinoma cells.

Yi-Ying Wu, Chin-Tung Hsieh, Ying-Ming Chiu, Shen-Chieh Chou, Jung-Ta Kao, Dong-Chen Shieh, Yi-Ju Lee

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 1 country.

Yi-Ying WuDepartment of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.ORCID 0000-0002-4205-4686
Chin-Tung HsiehDepartment of Pediatrics, Lo-Hsu Medical Foundation Lotung Poh-Ai Hospital, I-Lan, Taiwan.
Ying-Ming ChiuDepartment of Nursing, College of Medicine and Nursing, Hungkuang University, Taichung, Taiwan.
Shen-Chieh ChouDepartment of Biological Science and Technology, School of Medicine, China Medical University, Taichung, Taiwan.
Jung-Ta KaoGraduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
Dong-Chen ShiehDepartment of Nursing, College of Medicine and Nursing, Hungkuang University, Taichung, Taiwan.
Yi-Ju LeeInstitute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
China Medical University · TWChanghua Christian Hospital · TWChung Shan Medical University · TWHungkuang University · TWLuodong Poh-Ai Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis has been reported in some cancer cells, including AGS human gastric adenocarcinoma cells. Reducing this resistance might shed light on the treatment of human gastric adenocarcinoma. In this study, we examined whether glycogen synthase kinase-3 (GSK-3) inhibitors can restore TRAIL responsiveness in gastric adenocarcinoma cells. The effect of two GSK-3 inhibitors, SB-415286, and LiCl, on apoptosis signaling of TRAIL in human gastric adenocarcinoma cell lines and primary gastric epithelial cells was analyzed. Both inhibitors can sensitize gastric adenocarcinoma cells, but not primary gastric epithelial cells, to TRAIL-induced apoptosis by increasing caspase-8 activity and its downstream signal transmission. Adding p53 siRNA can downregulate GSK-3 inhibitor-related sensitization to TRAIL-induced apoptosis and caspase-3 activity. GSK-3 inhibitors strongly activate the phosphorylation of JNK. Inhibition of JNK leads to earlier and more intense apoptosis, showing that the activation of JNK may provide anti-apoptotic equilibrium of pro-apoptotic cells. Our observations indicate that GSK-3 inhibitors can sentize AGS gastric adenocarcinoma cells to TRAIL-induced apoptosis. Therefore, in certain types of gastric adenocarcinoma, GSK-3 inhibitor might enhance the antitumor activity of TRAIL and mightbe a promising candidate for the treatment of certain types of gastric adenocarcinoma.

Indexed as

AdenocarcinomaAminophenolsApoptosisCell Line, TumorDrug Screening Assays, AntitumorGlycogen Synthase Kinase 3HumansLithium ChlorideMaleimidesProtein Kinase InhibitorsRNA, Small InterferingStomach NeoplasmsTNF-Related Apoptosis-Inducing LigandTumor Suppressor Protein p533-(3-chloro-4-hydroxyphenylamino)-4-(4-nitrophenyl)-1H-pyrrole-2,5-dioneAminophenolsGlycogen Synthase Kinase 3Lithium ChlorideMaleimidesProtein Kinase InhibitorsRNA, Small InterferingTNF-Related Apoptosis-Inducing LigandTNFSF10 protein, humanTP53 protein, humanTumor Suppressor Protein p53

Identifiers

PMID30557366
PMCPMC6296518
OpenAlexW2904062907

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.