ArticleScientific reports2018
Exploring the rumen fluid metabolome using liquid chromatography-high-resolution mass spectrometry and Molecular Networking.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Diagnosing Anaerobic Digesters' Function and Performance: From Meta-Omics to Integrated Meta-Omics Analyses.Environmental microbiology reports · 2026Review
- Antimicrobial Peptides Improved Growth Performance by Intervening Ileac Microorganisms and Metabolites in Holstein Steers.Probiotics and antimicrobial proteins · 2026Article
- Effects of Composite Antimicrobial Peptides on Colonic Microbiota and Barrier Integrity in Holstein Steers.Probiotics and antimicrobial proteins · 2026Article
- Responsive changes of rumen metabolome and fatty acid profiles in blood and milk in dairy cows with different sorting behavior.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Fecal metabolome in Nelore bulls fed pre- and post-feedlot diets.Tropical animal health and production · 2025Article
- Alteration of Cecal Microbiota by Antimicrobial Peptides Enhances the Rational and Efficient Utilization of Nutrients in Holstein Bulls.Probiotics and antimicrobial proteins · 2025Article
- Enhancing Holstein steers growth performance: oregano essential oil's impact on rumen development, functionality and microorganism.Animal microbiome · 2025Article
- Oregano essential oil enhanced body weight and well-being by modulating the HPA axis and 23-nordeoxycholic acid of cecal microbiota in Holstein steers under cold stress.Animal microbiome · 2025Article
- Insights into rutin and quercetin biotransformations in ruminants revealed by molecular networking.Journal of animal science · 2025Article
- The Effects of Mixed Inoculum Storage Time on In Vitro Rumen Fermentation Characteristics, Microbial Diversity, and Community Composition.Animals : an open access journal from MDPI · 2024Article
- The Impact of Varying Pasture Levels on the Metabolomic Profile of Bovine Ruminal Fluid.Metabolites · 2024Article
- Castration alters the ileum microbiota of Holstein bulls and promotes beef flavor compounds.BMC genomics · 2024Article
- Ruminal Microbiome Differences in Angus Steers with Differing Feed Efficiencies during the Feedlot Finishing Phase.Microorganisms · 2024Article
- Hybridization promotes growth performance by altering rumen microbiota and metabolites in sheep.Frontiers in veterinary science · 2024Article
- Non-targeted multimodal metabolomics data from ovine rumen fluid fractions.Microbiology resource announcements · 2023Article
- Characterization of rumen microbiome and metabolome from oro-esophageal tubing and rumen cannula in Holstein dairy cows.Scientific reports · 2023Article
- Antimicrobial peptides act on the rumen microbiome and metabolome affecting the performance of castrated bulls.Journal of animal science and biotechnology · 2023Article
- Cytotoxic and chemomodulatory effects of Phyllanthus niruri in MCF-7 and MCF-7Scientific reports · 2023Article
- Buffalo milk and rumen fluid metabolome are significantly affected by green feed.Scientific reports · 2023Article
- Polyphenols for Livestock Feed: Sustainable Perspectives for Animal Husbandry?Molecules (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rumen primary and secondary metabolite content is intimately related to its community of bacteria, protozoa, fungi, archaea and bacteriophages, ingested feed and the host. Despite the myriad of interactions and novel compounds to be discovered, few studies have explored the rumen metabolome. Here, we present the first study using ultra-high performance liquid chromatography tandem mass-spectrometry and Molecular Networking approach, and various extraction methods on the cell-free rumen fluid of a non-lactating Holstein cow. Putative molecules were annotated based on accurate fragmentation matching the Global Natural Products Social Molecular Networking library, public spectral libraries, or annotated manually. The combination of five extraction methods resulted on 1,882 molecular features observed. Liquid-liquid extraction resulted on the highest molecular features abundance, 1,166 (61.96% of total). Sixty-seven compounds were annotated using Global Natural Products Social Molecular Networking library and public libraries, such as hydrocinnamic and azelaic acid, and monensin. Only 3.56% of molecular features (67) observed had positive match with available libraries, which shows the potential of the rumen as reservoir of novel compounds. The use of untargeted metabolomics in this study provided a snapshot of the rumen fluid metabolome. The complexity of the rumen will remain long unknown, but the use of new tools should be encouraged to foster advances on the rumen metabolome.
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