ArticleParasites & vectors2019
Screening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV-2 and their effect on replication of DENV-2 in C6/36 cells.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Comprehensive Larval microRNA Atlas ofInternational journal of molecular sciences · 2026Article
- Expression of endogenous Anopheles gambiae microRNAs using an Anopheles gambiae densovirus (AgDNV) intronic expression system.Parasites & vectors · 2025Article
- Response of the mosquito immune system and symbiotic bacteria to pathogen infection.Parasites & vectors · 2024Review
- Review
- Aedes albopictus salivary adenosine deaminase is an immunomodulatory factor facilitating dengue virus replication.Scientific reports · 2023Article
- Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease.Scientific reports · 2023Article
- The Involvement of Atlastin in Dengue Virus andMicrobiology spectrum · 2022Article
- Revised Annotation and Characterization of NovelBiology · 2022Article
- Review
- Profiling of MicroRNAs in Midguts ofFrontiers in genetics · 2021Article
- Tropical Medicine in China: Bibliometric Analysis Based on Web of Science (2010-2019).Journal of tropical medicine · 2021Article
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Evaluation of Exosomal miRNA in Blood as a Potential Diagnostic Biomarker for Human Non-Small Cell Lung Cancer.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundThe mosquito Aedes albopictus is an important vector for dengue virus (DENV) transmission. The midgut is the first barrier to mosquito infection by DENV, and this barrier is a critical factor affecting the vector competence of the mosquito. However, the molecular mechanism of the interaction between midgut and virus is unknown.
resultsSix small libraries of Ae. albopictus midgut RNAs were constructed, three of which from mosquitoes that were infected with DENV-2 after feeding on infected blood, and another three that remained uninfected with DENV-2 after feeding on same batch of infected blood. A total of 46 differentially expressed miRNAs were identified of which 17 significant differentially expressed miRNAs were selected. Compared to microRNA expression profiles of mosquitoes that were uninfected with DENV-2, 15 microRNAs were upregulated and two were downregulated in mosquitoes that were infected with DENV-2. Among these differentially expressed microRNAs, miR-1767, miR-276-3p, miR-4448 and miR-622 were verified by stem-loop qRT-PCR in samples from seven-day-infected and uninfected midguts and chosen for an in vitro transient transfection assay. miR-1767 and miR-276-3p enhanced dengue virus replication in C6/36 cells, and miR-4448 reduced dengue virus replication.
conclusionsTo our knowledge, this study is the first to reveal differences in expression levels between mosquitoes infected and uninfected with DENV-2 after feeding on an infected blood meal. It provides useful information on microRNAs expressed in the midgut of Aedes albopictus after exposure to the virus.
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